Protein kinase A regulates production of virulence determinants by the entomopathogenic fungus, Metarhizium anisopliae
Protein kinase A regulates production of virulence determinants by the entomopathogenic fungus, Metarhizium anisopliae
复制标题
DOI：
10.1016/j.fgb.2008.12.001
复制
发表时间：
2009-03-01
影响因子：
3
通讯作者：
Leger, Raymond St.
中科院分区：
文献类型：
--
作者：
Fang, Weiguo;Pava-Ripoll, Monica;Leger, Raymond St.
元中元素是研究昆虫真菌发病机理的模型系统。通过破坏I类PKA催化亚基基因（MAPKA1）来研究CAMP信号转导在毒力中的作用。 PKA突变体（Delta MAPKA1）显示出生长降低，毒力降低。 PKA对于分化感染结构（appressoria）是可分配的，但是由于胆压和甘油中的野生型，差异延迟，appressoria延迟了，Delta Mapka1的appressoria是有缺陷的，但在丙氨酸上发芽的速度相似。 Delta Mapka1对塑料表面的分生物粘附和appressorium形成被葡萄糖完全抑制为唯一的营养源。甘油或丙氨酸不会抑制塑料对塑料的粘附，但会延迟appressorium的形成。 Delta Mapka1显示出对氧化剂二敏氧化剂的耐受性降低，但对H2O2和甲基毒素剂的耐受性降低。 Delta MAPKA1和野生型应变的比较转录组分析表明，PKA负责上调昆虫角质层诱导的基因的大约三分之一，包括负责appressoria和渗透PEG的那些子集，表皮降解，养分，pHENIENT，PHENECITION，PHIPID CONSUTION，PHIPID SYTHESOS，CYLECES COLTATES，CYTOSS CYTRECT和CYTECTONT和CYTECTONT和CYTECTONT和CYTECTONT和CYTECT，CYTK CYTECT和CYTECT。但是，不需要PKA来表达产生毒素的基因。因此，我们得出结论，MAPKA1是感测与宿主相关的刺激和这些信号的转导所必需的，以调节许多感染过程。由Elsevier Inc.出版
Metarhizium anisopliae is a model system for studying insect fungal pathogenesis. The role of cAMP signal transduction in virulence was studied by disrupting the class I PKA catalytic subunit gene (MaPKA1). The PKA mutant (Delta MaPKA1) showed reduced growth and greatly reduced virulence. PKA was dispensable for differentiation of infection structures (appressoria), but differentiation was delayed and the appressoria were defective because of reduced turgor pressure, Delta MaPKA1 germinated at similar rates as the wild type in glucose and glycerol, but germination was delayed on alanine. Conidial adhesion and appressorium formation by Delta MaPKA1 against a plastic surface was fully inhibited with glucose as sole nutrient source. Adhesion to plastic was not inhibited with glycerol or alanine, but appressorium formation was delayed. Delta MaPKA1 showed reduced tolerance to the oxidative agent diamide, but not to H2O2 and methyl-viologen. Comparative transcriptome analysis of Delta MaPKA1 and the wild type strain showed that PKA is responsible for up-regulating approximately one-third of the genes induced by insect cuticle, including subsets of those responsible for differentiation of appressoria and penetration pegs, cuticle degradation, nutrient acquisition, pH regulation, lipid synthesis, cell cycle control and the cytoskeleton. PKA was not however required for expression of toxin-producing genes. We conclude therefore that MaPKA1 is required for sensing host-related stimuli and transduction of these signals to regulate many infection processes. Published by Elsevier Inc.