Insulin-like growth factors (IGFs) and IGF-binding proteins in human skin interstitial fluid.
Insulin-like growth factors (IGFs) and IGF-binding proteins in human skin interstitial fluid.
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人类皮肤间质液中的胰岛素样生长因子 (IGF) 和 IGF 结合蛋白。
DOI：
10.1210/jcem.80.10.7559878
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
J. Holly
中科院分区：
文献类型：
--
作者：
S. Xu;S. Cwyfan;J. V. D. van der Stappen;J. Sansom;J. Burton;M. Donnelly;J. Holly
尽管对循环和体液中的胰岛素样生长因子(IGF)/IGF结合蛋白（IGFBP）系统进行了广泛的研究，但在间质液中尚无相关信息。我们比较了10名健康志愿者血液循环中的IGF/IGFBP系统与通过负压人工培养的水疱液体中的IGF/IGFBP系统。IGFBP-1、-2、-3和-4均存在于水疱液中，但浓度远低于匹配血清。RIA测定的IGF-I、IGF-II和IGFBP-3水平分别为血清水平的18%、14%和16%。快速蛋白液相色谱法显示，在150千道尔顿复合物和50千道尔顿复合物中，IGF-I和IGFBP-3分别约为匹配血清中相应峰的13%和37%。与血清相比，水泡液中的IGFBP-3主要以改良的29千道尔顿形式存在，并且IGFBP-3蛋白酶活性增加。因此，尽管IGF在组织液中的浓度远低于循环中的浓度，但更大比例的IGF以更容易与组织受体相互作用的形式存在。水疱液似乎代表了生理间质液，并可能为研究间质环境中生长因子的生理和病理生理提供模型。
Despite extensive investigation of the insulin-like growth factor (IGF)/IGF-binding protein (IGFBP) system in the circulation and body fluids, there is no information on this in interstitial fluid. We have compared the IGF/IGFBP system in the circulation with that in fluid obtained from blisters artificially raised by negative pressure in 10 healthy volunteers. IGFBP-1, -2, -3, and -4 were all found in blister fluid, but in concentrations much lower than those in matched serum. The IGF-I, IGF-II, and IGFBP-3 levels measured by RIA were 18%, 14%, and 16% of those in serum, respectively. Fast protein liquid chromoatography showed that both IGF-I and IGFBP-3 in 150- and 50-kilodalton complexes were approximately 13% and 37%, respectively, of the corresponding peaks found in matched serum. Compared to that in serum, the IGFBP-3 in the blister fluid was predominantly in a modified 29-kilodalton form, and there was increased activity of an IGFBP-3 protease. Therefore, although IGF concentrations are much lower in interstitial fluid than in the circulation, a greater proportion of this IGF is in forms more readily available for interaction with tissue receptors. The blister fluid appears to represent physiological interstitial fluid and may provide a model for studying the physiology and pathophysiology of growth factors in the interstitial environment.
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影响因子：
5.8
作者：
GIUDICE, LC;FARRELL, EM;ROSENFELD, RG
通讯作者：
ROSENFELD, RG
脑脊液和羊水中胰岛素样生长因子结合蛋白的结构和免疫学比较。
DOI：
10.1210/jcem-68-3-638
发表时间：
1989
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Rosenfeld,RG;Pham,H;Conover,CA;Hintz,RL;Baxter,RC
通讯作者：
Baxter,RC
人体生物体液中胰岛素样生长因子结合蛋白 3 的测量和表征：放射免疫测定法和配体印迹之间的差异。
影响因子：
4.8
作者：
Gargosky,SE;Pham,HM;Wilson,KF;Liu,F;Giudice,LC;Rosenfeld,RG
通讯作者：
Rosenfeld,RG
选择性手术后诱导胰岛素样生长因子结合蛋白 3 蛋白水解。
DOI：
10.1210/jcem.75.2.1379257
发表时间：
1992
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Davenport,ML;Isley,WL;Pucilowska,JB;Pemberton,LB;Lyman,B;Underwood,LE;Clemmons,DR
通讯作者：
Clemmons,DR
人精浆中胰岛素样生长因子结合蛋白 2 (IGF-BP-2) 和低分子量 IGF-BP 的鉴定。
DOI：
10.1210/jcem-70-2-551
发表时间：
1990
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Rosenfeld,RG;Pham,H;Oh,Y;Lamson,G;Giudice,LC
通讯作者：
Giudice,LC