Characterization of cereal bare patch isolates of Rhizoctonia solani by random amplified polymorphic DNA analysis
Characterization of cereal bare patch isolates of Rhizoctonia solani by random amplified polymorphic DNA analysis
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通过随机扩增多态性 DNA 分析表征立枯丝核菌谷物裸斑分离株
DOI：
10.1111/j.1365-3059.1995.tb02740.x
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发表时间：
1995
期刊：
影响因子：
2.7
通讯作者：
P. O'Brien
中科院分区：
文献类型：
--
作者：
Huaan Yang;K. Sivasithamparam;J. E. Barton;P. O'Brien
利用RAPD-PCR技术对西澳大利亚州纽德盖特和埃斯佩兰斯两个地点的谷物和牧草裸地的枯丝核菌进行了鉴定。所有分离株均属于吻合组8、果胶酶谱组1-1或2。果胶酶谱可通过RAPD-PCR进行鉴定。在Newdegate的高毒力和弱毒分离株之间，以及Newdegate不同斑块的分离株之间，RAPD-PCR模式没有差异。Newdegate的分离株与Esperance的分离株和南澳大利亚不同地区的分离株相同。
RAPD-PCR was used to characterize isolates of Rhizoctonia solani from bare patches in cereal and pasture crops at two locations in Western Australia, Newdegate and Esperance. All of the isolates belonged to anastomosis group 8, pectic zymogram group 1-1 or 2. The pectic zymogram assignment could be confirmed by RAPD-PCR. There was no difference in RAPD-PCR pattern between highly virulent and weakly virulent isolates at Newdegate, or between isolates from different patches at Newdegate. The Newdegate isolates were identical to isolates from Esperance, and to isolates from various locations in South Australia.