Augmented production of chemokines (monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) and MIP-1beta) in patients with systemic sclerosis: MCP-1 and MIP-1alpha may be involved in the development of pulmonary fibrosis.
Augmented production of chemokines (monocyte chemotactic protein-1 (MCP-1), macrophage inflammatory protein-1alpha (MIP-1alpha) and MIP-1beta) in patients with systemic sclerosis: MCP-1 and MIP-1alpha may be involved in the development of pulmonary fibrosis.
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系统性硬化症患者趋化因子（单核细胞趋化蛋白-1 (MCP-1)、巨噬细胞炎症蛋白-1α (MIP-1α) 和 MIP-1β）的产生增加：MCP-1 和 MIP-1α 可能参与了这一过程
DOI：
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发表时间：
1999
影响因子：
4.6
通讯作者：
K. Takehara
中科院分区：
文献类型：
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作者：
M. Hasegawa;S. Sato;K. Takehara
为了确定趋化因子在系统性硬化症（SSc）发病机制中的作用，我们检测了SSc患者血清MCP-1、MIP-1 α和MIP-1 β的水平、外周血单核细胞（PBMC）自发产生的MCP-1、MIP-1 α和MIP-1 β以及受累皮肤的组织学分布。用ELISA法检测了58例SSc患者和20例正常人血清中趋化因子的水平。这些趋化因子在PBMC培养上清液中的水平也通过ELISA测量。与正常对照组相比，SSc患者血清MCP-1、MIP-1 α和MIP-1 β水平和PBMC自发产生水平显著升高。血清MCP-1和MIP-1 α水平升高与肺纤维化的存在显著相关。用抗MCP-1单克隆抗体免疫组化法检测SSc皮肤中MCP-1的表达。MCP-1在SSc患者的表皮、炎症单核细胞和血管内皮细胞中强烈表达，但在任何对照皮肤中均不表达。单核细胞和内皮细胞MCP-1的表达与SSc的早期发病密切相关。因此，MCP-1，MIP-1 α和MIP-1 β可能参与疾病过程，可能是通过增加白细胞迁移到SSc中受影响的组织中。MCP-1和MIP-1 α可能在SSc肺纤维化的发生发展中起重要作用。
To determine the role of chemokines in the pathogenesis of systemic sclerosis (SSc), we examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution in the affected skin, of MCP-1, MIP-1alpha and MIP-1beta in SSc patients. Serum levels of these chemokines were examined by ELISA in 58 patients with SSc and 20 normal controls. The levels of these chemokines in culture supernatants from PBMC were also measured by ELISA. Serum levels and spontaneous production levels by PBMC of MCP-1, MIP-1alpha, and MIP-1beta were significantly elevated in patients with SSc compared with normal controls. Elevated serum levels of MCP-1 and MIP-1alpha significantly correlated with the presence of pulmonary fibrosis. MCP-1 expression in the skin of SSc was immunohistochemically examined using anti-MCP-1 MoAb. MCP-1 was strongly expressed in the epidermis, inflammatory mononuclear cells, and vascular endothelial cells in the sclerotic skin of SSc patients, but not expressed in any control skin. Furthermore, the MCP-1 expression in inflammatory mononuclear cells and endothelial cells significantly correlated with earlier onset of SSc. Thus, MCP-1, MIP-1alpha and MIP-1beta may be involved in the disease process, possibly by augmenting leucocyte migration into the affected tissues in SSc. Furthermore, MCP-1 and MIP-1alpha may play an important role in the development of pulmonary fibrosis in SSc.