Prospects of using DNA barcoding for species identification and evaluation of the accuracy of sequence databases for ticks (Acari: Ixodida)
Prospects of using DNA barcoding for species identification and evaluation of the accuracy of sequence databases for ticks (Acari: Ixodida)
复制标题
使用 DNA 条形码进行蜱类物种鉴定和序列数据库准确性评估的前景（蜱虫：Ixodida）
DOI：
10.1016/j.ttbdis.2014.01.001
复制
发表时间：
2014-01-01
影响因子：
3.2
通讯作者：
Zhang, Bin
中科院分区：
文献类型：
--
作者：
Zhang, Rui L.;Zhang, Bin
蜱是牲畜疾病和寄生虫的重要媒介。传统上蜱的种类鉴定主要基于形态特征，但往往受到样品条件的限制，且标本间变异较小，因此需要一种快速、可靠的鉴定方法。 DNA 条形码使用线粒体基因细胞色素氧化酶 C 亚基 I (COI) 的标准片段来识别物种，并已成功用于许多分类单元。在这项研究中，我们将 DNA 条形码应用于蜱种。 K2P距离显示大多数种间差异超过8%，而种内距离通常低于2%。然而，12 个物种的种内距离出乎意料地高。 ABGD 分组结果表明这些物种的序列应分为 2 个或更多组。在Hyalomma marginatum，Hy 序列之间发生了一些特殊的聚类。元宝和Hy。 dromedarii、Amblyomma testudinarium 和 A. pattoni、Rhipicephalus sanguineus 和 R. pumilio、Haemaphysalis parva 和 Ha。 concinna、Ixodes asanumai 和 I. nipponensis。此外，226个未命名序列被分配给已知物种或构成不同的组，并且所有这些组的K2P距离均小于2%。总之，我们的研究表明 DNA 条形码是识别蜱物种的有用工具，需要进一步的工作来揭示一些有问题的属中模糊的物种界限。 (C) 2014 年爱思唯尔有限公司。版权所有。
Ticks are important vectors of disease and parasites of livestock. Species identification of ticks has been traditionally based on morphological characters, which is usually limited by the condition of samples and little variation among specimens, so a rapid and reliable identification method is needed. DNA barcoding uses a standard fragment of the mitochondrial gene cytochrome oxidase c subunit I (COI) to identify species and has been successfully used in many taxa. In this study, we applied DNA barcoding to tick species. K2P distances showed that most interspecific divergences exceed 8%, while intraspecific distances were usually lower than 2%. However, intraspecific distances of 12 species were unexpectedly high. ABGD grouping results demonstrated that sequences of these species should be divided into 2 or more groups. And some exceptional clustering occurred among sequences of Hyalomma marginatum, Hy. truncatum, and Hy. dromedarii, Amblyomma testudinarium and A. pattoni, Rhipicephalus sanguineus and R. pumilio, Haemaphysalis parva and Ha. concinna, Ixodes asanumai and I. nipponensis. Additionally, 226 unnamed sequences were assigned to known species or constituted different groups, and K2P distances of all these groups were less than 2%. In conclusion, our study demonstrated that DNA barcoding is a useful tool for the identification of tick species, and further work is needed to reveal ambiguous species delimitation in some problematic genera. (C) 2014 Elsevier GmbH. All rights reserved.