WHIRLY1 regulates HSP21.5A expression to promote thermotolerance in tomato
WHIRLY1 regulates HSP21.5A expression to promote thermotolerance in tomato
复制标题
WHIRLY1调节HSP21.5A表达促进番茄耐热性
DOI：
10.1093/pcp/pcz189
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发表时间：
2020
影响因子：
4.9
通讯作者：
Fanying Kong
中科院分区：
文献类型：
--
作者：
Kunyang Zhuang;Yangyang Gao;Zhuangbin Liu;Pengfei Diao;Na Sui;Qingwei Meng;Chen Meng;Fanying Kong
高温胁迫对植物生产力和作物产量构成了重大威胁。热休克因子诱导热休克蛋白(HSPs)是植物在热胁迫下的主要防御反应。在本研究中，我们克隆并分析了番茄(Solanum Lycopsicum)中的热胁迫诱导基因Whirly1(SlWHY1)。我们建立了不同的SLWHY1过表达(OE)和SLWHY1-RNA干扰(RNAi)系，以研究WHIRLY1在耐热性中的作用。与野生型(WT)相比，OE系在热胁迫下表现出较少的萎萎性，表现为膜稳定性和可溶性糖含量的增加以及活性氧(ROS)积累的减少。相反，SILWHY1表达受抑的RNAi品系在热胁迫下表现出相反的表型和相应的生理指标。与WT相比，热诱导基因SLHSP21.5A在OE系中上调，在RNAi系中下调，编码内质网定位的HSP。与对照相比，RNAi抑制SLHSP21.5A的表达还降低了膜的稳定性和可溶性糖含量，增加了ROS的积累。SLWHY1与SLHSP21.5A启动子中的激发子反应元件样元件结合，激活其转录。这些发现表明SLWHY1通过调节SLHSP21.5A的表达来提高番茄的耐热性。
Heat stress poses a major threat to plant productivity and crop yields. The induction of heat shock proteins (HSPs) by heat shock factors is a principal defense response of plants exposed to heat stress. In this study, we identified and analyzed the heat stress-inducedWhirly1(SlWHY1) gene in tomato (Solanum lycopersicum). We generated variousSlWHY1-overexpressing (OE) andSlWHY1-RNA interference (RNAi) lines to investigate the role of WHIRLY1 in thermotolerance. Compared with the wild type (WT), the OE lines showed less wilting, as reflected by their increased membrane stability and soluble sugar content and reduced reactive oxygen species (ROS) accumulation under heat stress. By contrast, RNAi lines with inhibitedSlWHY1expression showed the opposite phenotype and corresponding physiological indices under heat stress. The heat-induced geneSlHSP21.5A, encoding an endoplasmic reticulum-localized HSP, was upregulated in the OE lines and downregulated in the RNAi lines compared with the WT. RNAi-mediated inhibition ofSlHSP21.5Aexpression also resulted in reduced membrane stability and soluble sugar content and increased ROS accumulation under heat stress compared with the WT. SlWHY1 binds to the elicitor response element-like element in the promoter ofSlHSP21.5Ato activate its transcription. These findings suggest that SlWHY1 promotes thermotolerance in tomato by regulatingSlHSP21.5Aexpression.