Schwann cell expression of a major myelin glycoprotein in the absence of myelin assembly.
Schwann cell expression of a major myelin glycoprotein in the absence of myelin assembly.
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在没有髓磷脂组装的情况下，雪旺细胞表达主要髓磷脂糖蛋白。
DOI：
10.1073/pnas.81.6.1864
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发表时间：
1984
影响因子：
11.1
通讯作者：
Dyck,PJ
中科院分区：
文献类型：
--
作者：
Poduslo,JF;Berg,CT;Dyck,PJ
在没有髓磷脂组装的情况下，永久横断神经远端段的静止雪旺细胞产生基础水平的主要髓磷脂糖蛋白 P0。横切后 35 天，通过高分辨率十二烷基硫酸钠孔梯度电泳分离蛋白质后放射性碘化凝集素结合的放射自显影分析，以及掺入神经内膜切片后 [3H]岩藻糖和 [3H]甘露糖标记的糖蛋白电泳后的荧光分析，可以检测到低水平的 P0。横切神经中对 P0 的免疫反应性也可以通过针对 P0 的抗血清来证明，如通过电泳后直接“免疫覆盖”进行评估。这些结果表明，产生可检测量的髓磷脂特异性蛋白质和糖脂对来自适当轴突的持续信号的要求并不是绝对的。因此，施万细胞像少突胶质细胞一样，可以在没有神经元影响的情况下合成髓磷脂成分，尽管施万细胞组装髓磷脂和少突胶质细胞压缩髓磷脂可能需要来自神经元元件的信息。
Quiescent Schwann cells in the distal segment of the permanently transected nerve produced basal levels of the major myelin glycoprotein, P0, in the absence of myelin assembly. Low levels of P0 could be detected at 35 days after transection by autoradiographic analysis of radioiodinated lectin binding after protein separation by high-resolution sodium dodecyl sulfate pore gradient electrophoresis and by fluorographic analysis after electrophoresis of [3H]fucose- and [3H]mannose-labeled glycoproteins after incorporation into endoneurial slices. Immunoreactivity to P0 in the transected nerve could also be demonstrated with antisera against P0 as evaluated by direct "immune overlay" after electrophoresis. These results indicate that the requirement for continuing signals from appropriate axons to make detectable amounts of myelin-specific proteins and glycolipids is not absolute. Schwann cells, therefore, like oligodendrocytes, can synthesize myelin components in the absence of neuronal influence, although information from neuronal elements probably is required for myelin assembly by Schwann cells and for myelin compaction by oligodendrocytes.