Examining Galectin Binding Specificity Using Glycan Microarrays.
Examining Galectin Binding Specificity Using Glycan Microarrays.
复制标题
使用聚糖微阵列检查半乳糖凝集素结合特异性。
DOI：
10.1007/978-1-0716-2055-7_9
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Arthur,ConnieM
中科院分区：
文献类型：
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作者：
Stowell,SeanR;Rodrigues,LilianC;Dias-Baruffi,Marcelo;Cummings,RichardD;Arthur,ConnieM
聚糖结合蛋白（GBP）具有通过与高度可修饰的细胞表面聚糖相互作用来调节多种生物过程的独特能力。虽然许多研究证明了聚糖修饰对GBP识别和活性的影响，但聚糖结构的细微变化对GBP结合的相对贡献可能难以定义。为了克服在分析GBP-glycan相互作用中的局限性，最近的研究利用了含有数百个结构上确定的聚糖的聚糖微阵列平台。这些研究不仅提供了关于GBP-聚糖相互作用的重要信息，而且还导致了对半乳糖凝集素家族的结合特异性和生物活性的重要了解。我们将描述采用聚糖微阵列平台检查半乳糖凝集素-聚糖结合特异性和功能时使用的方法。
Glycan binding proteins (GBPs) possess the unique ability to regulate a wide variety of biological processes through interactions with highly modifiable cell surface glycans. While many studies demonstrate the impact of glycan modification on GBP recognition and activity, the relative contribution of subtle changes in glycan structure on GBP binding can be difficult to define. To overcome limitations in the analysis of GBP–glycan interactions, recent studies utilized glycan microarray platforms containing hundreds of structurally defined glycans. These studies not only provided important information regarding GBP–glycan interactions in general but have also resulted in significant insight into binding specificity and biological activity of the galectin family. We will describe the methods used when employing glycan microarray platforms to examine galectin–glycan binding specificity and function.