DnaA proteins of Escherichia coli and Bacillus subtilis: coordinate actions with single-stranded DNA-binding protein and interspecies inhibition during open complex formation at the replication origins.
DnaA proteins of Escherichia coli and Bacillus subtilis: coordinate actions with single-stranded DNA-binding protein and interspecies inhibition during open complex formation at the replication origins.
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DOI：
10.1016/s0378-1119(99)00009-8
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发表时间：
1999-03
期刊：
影响因子：
3.5
通讯作者：
M. Krause;W. Messer
中科院分区：
文献类型：
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作者：
M. Krause;W. Messer
DNA介导的解旋的AT-丰富的区域在大肠杆菌和枯草芽孢杆菌的复制起点进行了分析，在体外和没有单链DNA结合蛋白（SSB）。在SSB的存在下，解缠绕区域大了一个确定的碱基对数量。虽然起源的整体结构非常不同，但未缠绕区域的大小和结构相似。其中一种细菌oriC的解旋反应被另一种细菌的DnaA蛋白所抑制。类似地，杂交DNA A蛋白与交换DNA结合结构域是无活性的，抑制“开放复合物”的形成在两个起源。我们认为，抑制是由于非活性的混合配合物。
DnaA-mediated unwinding of the AT-rich region in the replication origins of Escherichia coli and Bacillus subtilis was analysed in vitro with and without single-stranded DNA-binding protein (SSB). In the presence of SSB, the unwound region was larger by a defined number of base pairs. Although the overall structure of the origins is very different, the size and structure of the unwound region were similar. The unwinding reaction at oriC of one organism was inhibited by DnaA protein of the other bacterium. Similarly, hybrid DnaA proteins with swapped DNA-binding domains were inactive and inhibitory to ‘open complex’ formation at both origins. We suggest that the inhibition is due to inactive mixed complexes.