Automated image analysis for diameters and branching points of cerebral penetrating arteries and veins captured with two-photon microscopy.
Automated image analysis for diameters and branching points of cerebral penetrating arteries and veins captured with two-photon microscopy.
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对用双光子显微镜捕获的脑穿通动脉和静脉的直径和分支点进行自动图像分析。
DOI：
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发表时间：
2014
影响因子：
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通讯作者：
K. Masamoto
中科院分区：
文献类型：
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作者：
Takuma Sugashi;K. Yoshihara;H. Kawaguchi;H. Takuwa;Hiroshi Ito;I. Kanno;Yukio Yamada;K. Masamoto
本研究旨在表征皮质微血管系统的三维（3D）形态（例如，穿透动脉和出现静脉），使用双光子显微镜和自动分析它们在小鼠皮质中的横截面直径和分支位置。我们观察到，动脉和静脉在皮质深度上具有可变的横截面直径。动脉（17 ± 5 μm）和静脉（15 ± 5 μm）的平均直径相似，在50-400 μm的深度范围内没有可检测到的差异。另一方面，动脉和静脉的分支数量在400 μm深处略有增加。动脉和静脉的每0.1 mm血管长度的平均分支数分别为1.7 ± 1.2和3.8 ± 1.6。该方法允许用双光子显微镜捕获的微血管图像的大体积数据的量化。这将有助于功能脑皮质微血管的形态计量学分析。
The present study was aimed to characterize 3-dimensional (3D) morphology of the cortical microvasculature (e.g., penetrating artery and emerging vein), using two-photon microscopy and automated analysis for their cross-sectional diameters and branching positions in the mouse cortex. We observed that both artery and vein had variable cross-sectional diameters across cortical depths. The mean diameter was similar for both artery (17 ± 5 μm) and vein (15 ± 5 μm), and there were no detectable differences over depths of 50-400 μm. On the other hand, the number of branches was slightly increased up to 400-μm depth for both the artery and vein. The mean number of branches per 0.1 mm vessel length was 1.7 ± 1.2 and 3.8 ± 1.6 for the artery and vein, respectively. This method allows for quantification of the large volume data of microvascular images captured with two-photon microscopy. This will contribute to the morphometric analysis of the cortical microvasculature in functioning brains.