Hippocampal gene expression in a rat model of depression after electroacupuncture at the Baihui and Yintang acupoints.
Hippocampal gene expression in a rat model of depression after electroacupuncture at the Baihui and Yintang acupoints.
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电针百会印堂穴后抑郁模型大鼠海马基因的表达
DOI：
10.4103/1673-5374.125333
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发表时间：
2014-01-01
影响因子：
6.1
通讯作者：
Chen L
中科院分区：
文献类型：
--
作者：
Duan D;Yang X;Ya T;Chen L
初步的基础研究和临床研究结果表明，电针治疗对抑郁症有积极的改善作用。然而，大多数研究的潜在机制是在单基因水平上，有几个关于在全基因组水平上的机制的报告。利用大鼠基因组芯片，我们分析了电针治疗后大鼠海马基因表达的变化。电针治疗可减轻模型大鼠的抑郁相关表现。利用基因芯片技术，我们证实电针百会、印堂穴调控了21个基因的表达。Real-time PCR结果显示，Vgf、Igf 2、Tmp 32、Loc 500373、Hif 1a、Folr 1、Nmb、Rtn等基因在抑郁症中表达上调或下调，电针治疗后其表达趋于正常。这些结果表明，电针百会和印堂通过调节特定基因的表达来调节抑郁症。
Preliminary basic research and clinical findings have demonstrated that electroacupuncture therapy exhibits positive effects in ameliorating depression. However, most studies of the underlying mechanism are at the single gene level; there are few reports regarding the mechanism at the whole-genome level. Using a rat genomic gene-chip, we profiled hippocampal gene expression changes in rats after electroacupuncture therapy. Electroacupuncture therapy alleviated depression-related manifestations in the model rats. Using gene-chip analysis, we demonstrated that electroacupuncture at Baihui (DU20) and Yintang (EX-HN3) regulates the expression of 21 genes. Real-time PCR showed that the genes Vgf, Igf2, Tmp32, Loc500373, Hif1a, Folr1, Nmb, and Rtn were upregulated or downregulated in depression and that their expression tended to normalize after electroacupuncture therapy. These results indicate that electroacupuncture at Baihui and Yintang modulates depression by regulating the expression of particular genes.