Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.
Effect of zinc ions on the biochemical behavior of simian virus 40 small-t antigen expressed in bacteria.
复制标题
锌离子对细菌表达的猿猴病毒40小-t抗原生化行为的影响。
DOI：
10.1128/jvi.66.3.1746-1751.1992
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发表时间：
1992
影响因子：
5.4
通讯作者：
Rundell,K
中科院分区：
文献类型：
--
作者：
Goswami,R;Turk,B;Enderle,K;Howe,A;Rundell,K
猿猴病毒40小t抗原含有10个半胱氨酸残基，其中6个被组织在两个CysXCysXXCys簇中。两个簇中单个Cys残基的突变或在这些簇之间发现的特定残基的突变导致动物细胞中蛋白质的显着不稳定性。蛋白质的不稳定性与细菌表达的突变蛋白结合锌离子的失败有关，锌离子的相互作用可以纯化大量的单体形式的小t抗原。
The simian virus 40 small-t antigen contains 10 cysteine residues, 6 of which are organized in two CysXCysXXCys clusters. Mutation of individual Cys residues in the two clusters or mutation of specific residues found between these clusters causes pronounced instability of the protein in animal cells. Protein instability correlates with failure of the bacterially expressed mutant proteins to bind zinc ions, an interaction which allows purification of large amounts of small-t antigen in monomeric form.