Role of egg sulfolipidimmobilizing protein 1 on mouse sperm-egg plasma membrane binding.
Role of egg sulfolipidimmobilizing protein 1 on mouse sperm-egg plasma membrane binding.
复制标题
卵硫脂固定蛋白 1 对小鼠精卵质膜结合的作用。
DOI：
10.1095/biolreprod61.3.749
复制
发表时间：
1999
影响因子：
3.6
通讯作者：
N. Tanphaichitr
中科院分区：
文献类型：
--
作者：
V. Ahnonkitpanit;D. White;S. Suwajanakorn;F. Kan;M. Namking;G. Wells;N. Kamolvarin;N. Tanphaichitr
我们已经证明，精子硫酰基调聚蛋白1 （SLIP1，分子量为68 kDa）是一种硫半乳糖基甘油脂（SGG）结合蛋白，在精子-透明带（ZP）相互作用中起重要作用。本研究的目的是定位SLIP1在卵细胞上的位置，并确定其在配子相互作用中的作用。免疫荧光和免疫蛋白A金电镜将SLIP1定位在卵质膜上。在体外配子结合中，在与精子共孵育之前，使用antiSLIP1 Fab预孵育无带卵子，结果显示精卵质膜结合明显呈剂量依赖性降低。用亲和纯化的抗slip1 IgG对鸡蛋进行预处理，得到了类似的结果。卵子SLIP1在精卵质膜结合中的重要意义进一步被证明，当带完整的卵子被抗SLIP1 IgG预处理后，体外受精的精子-卵子质膜结合减少（36-52%）。由于SLIP1在体外已被证明与SGG结合，我们研究了精子SGG可能通过卵子SLIP1参与精卵质膜结合的可能性。在与无带卵子共孵育前，用抗sgg Fab预处理精子导致精卵质膜结合的剂量依赖性降低。总的来说，这些发现强烈表明卵子SLIP1在精子-卵子质膜相互作用中的作用，这可能是通过它与精子SGG的结合。
We have shown that sperm sulfolipidimmobilizing protein 1 (SLIP1, molecular mass of 68 kDa), a sulfogalactosylglycerolipid (SGG)-binding protein, is significant in sperm-zona pellucida (ZP) interaction. The objective of this study was to localize SLIP1 on the egg and determine its role in gamete interaction. Immunofluorescence and immunoprotein A gold electron microscopy localized SLIP1 to the egg plasma membrane. In vitro gamete binding, using zona-free eggs preincubated with antiSLIP1 Fab before coincubation with sperm, showed a significant, dose-dependent decrease in sperm-egg plasma membrane binding. Similar results were obtained when affinity-purified antiSLIP1 IgG was used for egg pretreatment. The significance of egg SLIP1 in sperm-egg plasma membrane binding was further demonstrated by a decrease (36-52%) in in vitro fertilization when zona-intact eggs were pretreated with antiSLIP1 IgG. Since SLIP1 has been shown to bind SGG in vitro, we investigated the possibility that sperm SGG may participate in sperm-egg plasma membrane binding through egg SLIP1. Pretreatment of sperm with antiSGG Fab prior to coincubation with zona-free eggs resulted in a dose-dependent decrease in sperm-egg plasma membrane binding. Collectively, these findings strongly suggest a role for egg SLIP1 in sperm-egg plasma membrane interaction, which may be through its binding to sperm SGG.
DOI：
10.1139/o85-134
发表时间：
1985
期刊：
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire
影响因子：
--
作者：
Lingwood,CA
通讯作者：
Lingwood,CA