THE DERIVATION OF TWO DISTINCT ANAPHYLATOXIN ACTIVITIES FROM THE THIRD AND FIFTH COMPONENTS OF HUMAN COMPLEMENT
THE DERIVATION OF TWO DISTINCT ANAPHYLATOXIN ACTIVITIES FROM THE THIRD AND FIFTH COMPONENTS OF HUMAN COMPLEMENT
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从人类补体的第三和第五成分衍生出两种不同的过敏毒素活性
DOI：
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发表时间：
1968
影响因子：
15.3
通讯作者：
H. Müller
中科院分区：
文献类型：
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作者：
Charles G. Cochrane;H. Müller
过敏毒素活性来源于人C'5和C'3分子。这是在C'5通过与胰蛋白酶或EAC'4, oxy2a， 3相互作用的情况下实现的。从处理过的C'5中获得的平滑肌收缩物质被发现是一个大约9000 - 11000分子量的片段。抗组胺药抑制其作用。胰蛋白酶化的C'5也增加了豚鼠皮肤的血管通透性。用眼镜蛇毒蛋白和人血清β-球蛋白组成的C'3灭活复合物孵育人C'3，观察其对过敏毒素的活性。这种活性与从C'3分子中切割出来的片段有关，通过凝胶过滤技术确定，C'3分子的分子量在6000到15000之间。C'3转化酶在游离或细胞结合形式下也能从C'3获得类似的活性。C'5过敏毒素不能使豚鼠回肠对C'3和豚鼠过敏毒素的收缩能力交叉脱敏，反之亦然。以上几种方法制备的C’3过敏毒素对其他C’3衍生物均有交叉脱敏作用，但对豚鼠过敏毒素均无脱敏作用。
Anaphylatoxin activity was derived from both human C'5 and C'3 molecules. This was achieved in the case of C'5 by interaction with trypsin or with EAC'4, oxy2a, 3. The smooth muscle-contracting material obtained from the treated C'5 was found to be a fragment of approximately 9,000–11,000 molecular weight. Its action was inhibited with antihistamine. The trypsinized C'5 also increased vascular permeability in guinea pig skin. When human C'3 was incubated with C'3 inactivator complex, which consists of a cobra venom protein and a β-globulin of human serum, anaphylatoxin activity was observed. The activity was associated with a fragment cleaved from the C'3 molecule, having a molecular weight of between 6,000 and 15,000 as determined by gel filtration techniques. Similar activity was derived from C'3 by the C'3-converting enzyme in free or in cell-bound form. The C'5 anaphylatoxin failed to cross-desensitize guinea pig ileum to the contracting capacities of C'3 and guinea pig anaphylatoxin and vice versa. Anaphylatoxin prepared from C'3 by all methods mentioned above caused cross-desensitization to the other C'3 derivatives, but failed to desensitize to guinea pig anaphylatoxin.