PNA Arrays for miRNA Detection
PNA Arrays for miRNA Detection
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DOI：
10.1246/cl.2009.438
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发表时间：
2009-05-05
影响因子：
1.6
通讯作者：
Ohtsuki, Takashi
中科院分区：
文献类型：
--
作者：
Endoh, Tamaki;Kitamatsu, Mizuki;Ohtsuki, Takashi
利用微阵列技术分析microRNA（miRNAs）已被用于许多最近的生物学和医学研究。在这项研究中，肽核酸（PNAs）阵列的制备和它们的miRNA捕获能力进行了评估。我们发现10-mer PNA探针在靶向miRNA方面的灵敏度远高于相应的DNA探针和更长的（20-mer）DNA探针。
The profiling of microRNAs (miRNAs) using microarray technology has been used in many recent biological and medical studies. In this study, arrays of peptide nucleic acids (PNAs) were prepared and their miRNA capture abilities were evaluated. We found that the sensitivity of 10-mer PNA probes in targeting miRNAs was much higher than that of the corresponding DNA probes and also of longer (20-mer) DNA probes.