Population structure and introduction history of the pink bollworm, Pectinophora gossypiella, in China
Population structure and introduction history of the pink bollworm, Pectinophora gossypiella, in China
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中国红铃虫种群结构及引进历史
DOI：
10.1111/j.1570-7458.2008.00800.x
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发表时间：
2009-02-01
影响因子：
1.9
通讯作者：
Guo, Yu-Yuan
中科院分区：
文献类型：
--
作者：
Liu, Yu-Di;Wu, Kong-Ming;Guo, Yu-Yuan
棉铃虫Pectinophora Cotsypiella(Saunders)是一种可能产于印度-巴基斯坦的棉花害虫，于20世纪初入侵中国。中国棉疫霉被认为是通过运输棉花种子从巴基斯坦和美国不分青红皂白地引进的结果。我们验证了这一长期坚持的假设，并对13个微卫星座位上14个地点的527名个体进行了基因分型。我们用传统统计方法和贝叶斯方法对这些数据进行了分析。这些基因座在很大程度上是高度多态的。中国人群在6个基因座上的等位基因丰富度高于巴基斯坦人群和美国人群。所有14个群体都记录了显著的杂合子缺陷，零等位基因是导致这些缺陷的最可能的因素。两两F-ST估计表明，中国人、巴基斯坦人和美国人之间存在显著的分化，并且大多数中国人的群体内部存在结构。贝叶斯分析表明，中国人、美国人和巴基斯坦人组成了单独的聚类群，九个中国人群体被分成两个聚类群。等位基因频率分布表明，中国棉铃虫的专有和共享等位基因仅部分来源于巴基斯坦和美国群体。基于微卫星的遗传分析表明，中国棉铃虫种群的遗传来源是多源的。
The pink bollworm, Pectinophora gossypiella (Saunders) (Lepidoptera: Gelechiidae), a cotton pest probably native to Indo-Pakistan, invaded China at the beginning of the 20th century. Chinese P. gossypiella have been assumed to be the result of indiscriminate introductions from Pakistan and America by transport of cotton seed. We tested this long-held hypothesis and genotyped a total of 527 individuals from 14 sites at 13 microsatellite loci. We analyzed these data with traditional statistics as well as with Bayesian methods. The loci were, for the most part, highly polymorphic. The allelic richness of Chinese populations at six loci was greater than those of the Pakistani and American populations. Significant deficits of heterozygotes were recorded for all 14 populations, and null alleles were the most probable factor contributing to these deficits. Pairwise F-ST estimates showed that there was significant differentiation among the pooled Chinese, Pakistani, and American populations, and there was structure within most of the Chinese populations. The Bayesian analysis revealed that the combined Chinese, American, and Pakistani populations formed separate clusters, and the nine Chinese populations were divided into two clusters. Allelic frequency distributions showed that private and shared alleles within Chinese P. gossypiella were derived only partly from the Pakistani and American populations. The microsatellite-based genetic analyses suggested that the Chinese P. gossypiella populations originated from multiple sources.