Similarity of cruzin, an inhibitor of Trypanosoma cruzi neuraminidase, to high-density lipoprotein.
Similarity of cruzin, an inhibitor of Trypanosoma cruzi neuraminidase, to high-density lipoprotein.
复制标题
cruzin（克氏锥虫神经氨酸酶抑制剂）与高密度脂蛋白的相似性。
DOI：
10.1126/science.3120314
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Pereira,ME
中科院分区：
文献类型：
--
作者：
Prioli,RP;Ordovas,JM;Rosenberg,I;Schaefer,EJ;Pereira,ME
最近分离到一种寄生于克氏锥虫的神经氨酸酶特异性抑制剂，命名为cruzin。现在通过氨基酸同源性、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、免疫印迹分析和等电聚焦分析表明，cruzin与高密度脂蛋白相似。离子交换层析纯化的Cruzin和密度梯度离心分离的高密度脂蛋白对Cruzineuraminidase有相同程度的抑制作用。Cruzin或高密度脂蛋白使在脂蛋白耗尽的培养基中生长的克氏锥虫的繁殖率恢复正常，这表明它可能对寄生虫在自然界的生存很重要。
A specific inhibitor of the neuraminidase of the protozoan parasiteTrypanosoma cruziwas isolated recently and named cruzin. It is now shown that cruzin is similar to high-density lipoprotein by amino acid homology, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, by immunoblot analysis, and by isoelectric focusing. Cruzin purified by ion exchange chromatography and high-density lipoprotein isolated by density gradient ultracentrifugation inhibitedTrypanosoma cruzineuraminidase to the same extent. Cruzin or high-density lipoprotein restores to normal the decreased multiplication rate ofTrypanosoma cruziepimastigotes grown in a medium depleted of lipoproteins, suggesting that it may be important for survival of the parasite in nature.