Mammalian cell fusion III. A hela cell inducer of premature chromosome condensation active in cells from a variety of animal species
Mammalian cell fusion III. A hela cell inducer of premature chromosome condensation active in cells from a variety of animal species
复制标题
哺乳动物细胞融合 III.
DOI：
--
复制
发表时间：
1970
期刊：
影响因子：
--
通讯作者：
H. D. Hughes
中科院分区：
文献类型：
--
作者：
Robert T. Johnson;P. Rao;H. D. Hughes
通过将未受刺激的马淋巴细胞、牛精子、中国仓鼠卵巢细胞、鸡胚成纤维细胞和红细胞、非洲爪蟾肾和蚊子细胞中的每一种细胞类型与有丝分裂受阻的 HeLa 细胞融合，诱导出染色体过早浓缩 (PCC) 现象。因此，甚至可以从异源物种的非增殖细胞（例如鸡红细胞和牛精子）中观察染色体。
The phenomenon of premature chromosome condensation (PCC) is induced in unstimulated horse lymphocytes, bovine spermatozoa, Chinese hamster ovary cells, embryonic chick fibroblasts and erythrocytes, Xenopus kidney and mosquito cells by fusing each of these cell types with HeLa cells blocked in mitosis. Thus it becomes possible to visualize chromosomes even from non‐multiplying cells of heterologous species, such as, chick erythrocytes and bovine spermatozoa.