Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with Cucumber mosaic virus
Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with Cucumber mosaic virus
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DOI：
10.1111/mpp.12081
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发表时间：
2014-04-01
影响因子：
4.9
通讯作者：
Ohki, Satoshi T.
中科院分区：
文献类型：
--
作者：
Mochizuki, Tomofumi;Ogata, Yoshiyuki;Ohki, Satoshi T.
黄瓜花叶病毒（CMV）在接种的烟草植物中引起花叶病。外壳蛋白（CP）是CMV的主要毒力决定因子之一，CP第129位残基的氨基酸替换改变了感病烟草花叶组织中黄化的严重程度，如淡绿色黄化和白色黄化。在这项研究中，我们比较了褪绿组织的转录组感染野生型pepo株的CMV和两个菌株携带CP突变体与不同的褪绿严重程度。差异基因表达分析表明，CMV接种出现有症状的褪绿组织的转录表达谱的影响相似，只有不同的CMV之间的表达量不同。基因本体分析的生物过程和细胞组分的条款显示，许多核基因相关的非生物胁迫反应，包括镉，热，冷和盐的反应，上调，而叶绿体和光合作用相关基因（CPRGs）下调，在褪绿组织。有趣的是，CPRG下调的水平与黄化的严重程度相关。这些结果表明，CP突变控制CPRGs的阻遏水平和mRNA积累，这与黄化的诱导密切相关。
Cucumber mosaic virus (CMV) causes mosaic disease in inoculated tobacco plants. Coat protein (CP) is one of the major virulence determinants of CMV, and an amino acid substitution at residue 129 in CP alters the severity of chlorosis, such as pale green chlorosis and white chlorosis, in symptomatic tissues of mosaic leaves of infected tobacco. In this study, we compared the transcriptomes of chlorotic tissues infected with the wild-type pepo strain of CMV and two strains carrying CP mutants with diverse chlorosis severity. Differential gene expression analysis showed that CMV inoculation appeared to have similar effects on the transcriptional expression profiles of the symptomatic chlorotic tissues, and only the magnitude of expression differed among the different CMVs. Gene ontology analysis with biological process and cellular component terms revealed that many nuclear genes related to abiotic stress responses, including responses to cadmium, heat, cold and salt, were up-regulated, whereas chloroplast- and photosynthesis-related genes (CPRGs) were down-regulated, in the chlorotic tissues. Interestingly, the level of CPRG down-regulation was correlated with the severity of chlorosis. These results indicate that CP mutation governs the repression level and mRNA accumulation of CPRGs, which are closely associated with the induction of chlorosis.