The Effect of Phenazine-1-Carboxylic Acid on Mycelial Growth of Botrytis cinerea Produced by Pseudomonas aeruginosa LV Strain.
The Effect of Phenazine-1-Carboxylic Acid on Mycelial Growth of Botrytis cinerea Produced by Pseudomonas aeruginosa LV Strain.
复制标题
DOI：
10.3389/fmicb.2017.01102
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
de Oliveira AG
中科院分区：
文献类型：
--
作者：
Simionato AS;Navarro MOP;de Jesus MLA;Barazetti AR;da Silva CS;Simões GC;Balbi-Peña MI;de Mello JCP;Panagio LA;de Almeida RSC;Andrade G;de Oliveira AG
影响草莓、葡萄和西红柿的最重要的采后植物病原体之一是灰霉病，即灰霉病。这种真菌在孢子萌发条件良好之前一直处于潜伏状态，使感染控制变得困难，给整个生产链造成了巨大的损失。本研究旨在对铜绿假单胞菌LV菌株产生的吩嗪-1-羧酸(PCA)进行纯化和鉴定，并测定其对灰霉菌的抗真菌活性。生成的化合物用二氯甲烷提取，并通过层析过程。采用反相高效液相半制备法测定PCA的纯度。经核磁共振和电喷雾电离质谱仪确证了其结构。用干纸片法和最小抑菌浓度(MIC)法测定其抗真菌活性，并用扫描电子显微镜和共聚焦显微镜进行鉴定。结果表明，苦参碱对菌丝生长有抑制作用，最低抑菌浓度为25μg·m L-1。显微镜分析显示胞外多糖(EPS)的形成减少，显示菌丝扭曲和损坏。结果表明，PCA在控制灰霉病和抑制重要毒力因子EPS方面具有较高的潜力。这种天然化合物是防治灰霉病的一种潜在的采后选择。
One of the most important postharvest plant pathogens that affect strawberries, grapes and tomatoes is Botrytis cinerea, known as gray mold. The fungus remains in latent form until spore germination conditions are good, making infection control difficult, causing great losses in the whole production chain. This study aimed to purify and identify phenazine-1-carboxylic acid (PCA) produced by the Pseudomonas aeruginosa LV strain and to determine its antifungal activity against B. cinerea. The compounds produced were extracted with dichloromethane and passed through a chromatographic process. The purity level of PCA was determined by reversed-phase high-performance liquid chromatography semi-preparative. The structure of PCA was confirmed by nuclear magnetic resonance and electrospray ionization mass spectrometry. Antifungal activity was determined by the dry paper disk and minimum inhibitory concentration (MIC) methods and identified by scanning electron microscopy and confocal microscopy. The results showed that PCA inhibited mycelial growth, where MIC was 25 μg mL-1. Microscopic analysis revealed a reduction in exopolysaccharide (EPS) formation, showing distorted and damaged hyphae of B. cinerea. The results suggested that PCA has a high potential in the control of B. cinerea and inhibition of EPS (important virulence factor). This natural compound is a potential alternative to postharvest control of gray mold disease.