POLYMORPHISM OF T-CELL RECEPTOR GENES IN NASOPHARYNGEAL CARCINOMA
POLYMORPHISM OF T-CELL RECEPTOR GENES IN NASOPHARYNGEAL CARCINOMA
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DOI：
10.1002/ijc.2910560613
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发表时间：
1994-03-15
影响因子：
6.4
通讯作者：
CHAN, SH
中科院分区：
文献类型：
--
作者：
CHEN, Y;CHAN, SH
应用限制性片段长度多态性（RFLP）分析方法，对41例新加坡华人鼻咽癌患者和54例新加坡华人正常人的生殖系TCR基因进行了研究。用限制性内切酶BamHI、EcoRI、HindIII、Taq 1消化患者和正常人BLCL基因组DNA，用Southern印迹技术转移到尼龙膜上，与放射性标记的TCR cDNA探针杂交。当使用BamHI/V β 11的组合时，与对照组相比，NPC患者具有较低的25/25-kb等位基因模式频率和较高的20-kb片段频率。Taq 1/V β 8联合应用后，4.3/3.2-kb片段等位基因频率在总NPC中较低，但在HLA B46患者中尤其如此。结果提示，TCR限制性T淋巴细胞受体可能在鼻咽癌的发病机制中起重要作用。(C)1994 Wiley-Liss，Inc.
Germ-line TCR genes from 41 Singaporean Chinese patients with nasopharyngeal carcinoma and 54 Singapore Chinese normal subjects were investigated by restriction fragment length polymorphism (RFLP) analysis. Genomic DNA from BLCLs of patients and normal subjects was digested with the restriction endonucleases BamHI, EcoRI, HindIII, Taq1, transferred to nylon membrane by the Southern-blot technique and hybridized with radioactively labelled TCR cDNA probes. When the combination of BamHI/V beta 11 was used, NPC patients had a lower frequency of the 25/25-kb allelic pattern and a higher frequency of the 20-kb fragment compared to controls. With the combination of Taq1/V beta 8, the 4.3/3.2-kb fragment allelic frequency was lower in total NPC but particularly in patients with HLA B46. The result suggested that TCR restriction may be important in the pathogenesis of NPC. (C) 1994 Wiley-Liss, Inc.