"French fries"-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity
"French fries"-like luminescent metal organic frameworks for the fluorescence determination of cytochrome c released by apoptotic cells and screening of anticancer drug activity
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类“炸薯条”发光金属有机框架用于荧光测定凋亡细胞释放的细胞色素c及筛选抗癌药物活性
DOI：
10.1007/s00604-020-4207-x
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发表时间：
2020
影响因子：
5.7
通讯作者：
Zhao Peng
中科院分区：
文献类型：
--
作者：
Xie Ruirui;Liu Yanyan;Yang Peipei;Huang Lan;Zou Xun;Liu Jiamin;Ren Qingfan;Tao Jia;Zhao Peng
通过将Zn-Ag-In-S量子点封装到“薯条”状MIL-68（In）金属有机框架（ZAISQDs@MIL-68（In））中来制备发光金属有机框架。ZAISQD @MIL-68（In）的最大激发波长为370 nm，最大发射波长为620 nm。发现ZAISQDs@MIL-68（In）被细胞色素c（Cyt c）有效地猝灭，细胞色素c是早期细胞凋亡的重要生物标志物。其猝灭机制为Cyt c对ZAISQDs@MIL-68（In）的内过滤效应和动态猝灭以及MIL-68（In）的富集效应。基于以上优点，ZAISQDs@MIL-68（In）被开发为Cyt c的检测策略，在0.02 ~ 3.5 μM范围内，信号猝灭与浓度呈对数关系。线性方程为（F0-F）/F0= 0.5043 + 0.2678 × logcCyt c，检出限为8 nM。ZAISQDs@MIL-68（In）检测药物诱导凋亡细胞释放的Cyt c，并用于抗癌药物活性筛选。
A luminescent metal organic framework was prepared by encapsulating Zn–Ag–In–S quantum dots into “French fries”-like MIL-68(In) metal organic frameworks (ZAISQDs@MIL-68(In)). The ZAISQDs@MIL-68(In) had a maximum excitation wavelength at 370 nm and maximum emission wavelength at 620 nm. It was found that the ZAISQDs@MIL-68(In) was efficiently quenched by cytochrome c (Cyt c), which is an important biomarker of early cell apoptosis. The quenching mechanism was ascribed to be an inner filter effect and dynamic quenching of Cyt c towards the ZAISQDs@MIL-68(In), and the enrichment effect of MIL-68(In). Benefiting from the multiple advantages, ZAISQDs@MIL-68(In) was developed as an assay strategy of Cyt c with logarithmic relation between signal quenching and concentration in the range 0.02 to 3.5 μM. The linear equation was (F0–F)/F0= 0.5043 + 0.2678 × logcCyt cwith a detection limit of 8 nM. Cyt c released by drug induced apoptotic cells was determined by ZAISQDs@MIL-68(In), and this strategy has been utilized for the screening of anticancer drug activity.Graphical abstractSchematic representation of the synthesis of ZAISQDs@MIL-68(In) and its application for Cyt c and screening anticancer drug activity