Parathyroid hormone enhances fracture healing - A preliminary report
Parathyroid hormone enhances fracture healing - A preliminary report
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DOI：
10.1097/00003086-199909000-00033
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发表时间：
1999-09-01
影响因子：
4.2
通讯作者：
Einhorn, TA
中科院分区：
文献类型：
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作者：
Holzer, G;Majeska, RJ;Einhorn, TA
本研究验证了每日胃肠外给予甲状旁腺激素（1-34）促进骨折愈合的假设。20只3月龄雄性Sprague道利大鼠，每只重约400 g，进行闭合性单侧股骨中段骨折的制备。将动物分成两组，每组10只;动物每天皮下注射递送载体（0.9%盐水）或80 μ g/kg甲状旁腺激素。骨折后第21天，对动物实施安乐死，取出股骨并进行生物力学试验、骨密度测定（双能X射线吸收测定法、外周定量计算机断层扫描）和组织学检查。甲状旁腺激素治疗导致骨痂面积和强度的统计学显着增加。骨痂的组织学检查显示新骨形成量增加。在动物的体重或骨骼大小方面没有观察到差异。使用双能X线吸收测定法和外周定量计算机断层扫描获得的值表明，甲状旁腺激素治疗的骨折的密度增加与组织学表现和强度增加的结果一致，尽管这些骨密度变化没有达到统计学意义。这些结果表明，胃肠外给药甲状旁腺激素（1-34）可以增强或加速正常骨折愈合，并支持这种激素作为缓慢愈合的骨折的全身治疗进行临床试验的概念。
This investigation tested the hypothesis that daily parenterally administered parathyroid hormone (1-34) improves fracture healing. Twenty, 3-month-old, male Sprague Dawley rats weighing approximately 400 g each, underwent the production of closed, unilateral middiaphyseal femoral fractures. Animals were divided into two groups of 10; the animals received either a daily subcutaneous injection of delivery vehicle (0.9 % saline) or 80 mu g/kg parathyroid hormone. On Day 21 after fracture the animals were euthanized, the femurs were removed and subjected to biomechanical testing, bone densitometry (dual energy xray absorptiometry, peripheral quantitative computed tomography), and histologic examination. Treatment with parathyroid hormone resulted in statistically significant increases in callus area and strength. Histologic examination of the calluses showed an increase in the amount of new bone formed. No differences were observed in the weights of the animals: or the sizes of the bones. Values obtained using dual energy xray absorptiometry and peripheral quantitative computed tomography indicate an increase in density in the parathyroid hormone treated fractures consistent with the histologic appearance and the findings of increased strength, although these bone density changes did not achieve statistical significance. These results suggest that parenterally administered parathyroid hormone (1-34) may enhance or accelerate normal fracture healing and support the concept that this hormone be tested clinically as a systemic treatment for fractures that are slow to heal.