Phospholipid biosynthesis in human platelets. The acylation of lyso-platelet-activating factor.
Phospholipid biosynthesis in human platelets. The acylation of lyso-platelet-activating factor.
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人血小板中的磷脂生物合成。
DOI：
10.1042/bj2250723
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Silver,MJ
中科院分区：
文献类型：
--
作者：
McKean,ML;Silver,MJ
人血小板酰基辅酶a:1-烷基- cn -甘油-3-磷酸胆碱酰基转移酶是膜结合的，pH最适值为7.5，对1 mM-Mg2+不敏感，1 mM-Ca2+抑制，1 mM-EDTA轻微刺激。在150微米的1-烷基-2-酰基-sn-甘油-3-磷酸胆碱和20微米的不饱和脂肪酰基辅酶a的最大形成。不饱和脂肪酸酰基向1-烷基-sn-甘油-3-磷酸胆碱的转移比向1-酰基-sn-甘油-3-磷酸胆碱的转移慢3-14倍。亚油酸和花生四烯酸的辅酶a酯是血小板磷脂中常见的两种不饱和脂肪酰基，是首选的脂肪酰基供体。
Acyl-CoA:1-alkyl-sn-glycero-3-phosphocholine acyltransferase of human platelets is membrane-bound, has a pH optimum of 7.5, is insensitive to 1 mM-Mg2+, is inhibited by 1 mM-Ca2+, and is stimulated slightly by 1 mM-EDTA. Maximal formation of 1-alkyl-2-acyl-sn-glycero-3-phosphocholine is observed at 150 microM-1-alkyl-sn-glycero-3-phosphocholine and 20 microM unsaturated fatty acyl-CoA. The transfer of unsaturated fatty acyl groups to 1-alkyl-sn-glycero-3-phosphocholine is 3-14 times slower than to 1-acyl-sn-glycero-3-phosphocholine. The CoA esters of linoleate and arachidonate, two unsaturated fatty acyl groups commonly found in platelet phospholipids, are the preferred fatty acyl group donors.