Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells.
Lipocalin-2 is associated with radioresistance in oral cancer and lung cancer cells.
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DOI：
10.3892/ijo.2013.1815
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发表时间：
2013-04
影响因子：
5.2
通讯作者：
M. Shiiba;Kengo Saito;K. Fushimi;T. Ishigami;K. Shinozuka;D. Nakashima;Y. Kouzu;Hirofumi Koike;A. Kasamatsu;Yosuke Sakamoto;K. Ogawara;K. Uzawa;Y. Takiguchi;H. Tanzawa
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文献类型：
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作者：
M. Shiiba;Kengo Saito;K. Fushimi;T. Ishigami;K. Shinozuka;D. Nakashima;Y. Kouzu;Hirofumi Koike;A. Kasamatsu;Yosuke Sakamoto;K. Ogawara;K. Uzawa;Y. Takiguchi;H. Tanzawa
本研究的目的是确定一个靶分子，可以预测放射治疗在口腔鳞状细胞癌（OSCC）的疗效。我们使用DNA微阵列分析来确定X射线照射后基因表达的差异。我们比较了X射线（8戈伊）照射的Ca 9 -22细胞（OSCC衍生的细胞系）和未照射的Ca 9 -22细胞之间的基因表达谱。共鉴定了167个基因，其表达水平比X射线照射诱导的高2倍。X射线照射后，Lipocalin-2（LCN 2）的表达增加幅度最大，并且使用Ingenuity Pathway Analysis工具将其归类为具有癌症相关功能的网络。通过实时定量逆转录酶-聚合酶链反应（qRT-PCR）分析验证LCN 2 mRNA表达上调。当用小干扰RNA敲低OSCC细胞（Ca 9 -22和HSC-2）和肺癌细胞（A549）中的LCN 2基因时，这些细胞的放射敏感性增强。我们的研究结果表明，LCN 2的过度表达可能与口腔癌和肺癌细胞的放射抗性有关，LCN 2表达水平可用于预测放射抗性。因此，调节LCN 2的表达或功能可以增强放射反应，导致放射治疗的有利结果。
The aim of the present study was to identify a target molecule that could predict the efficacy of radiotherapy in oral squamous cell carcinoma (OSCC). We used DNA microarray analysis to identify differences in gene expression after X-ray irradiation. We compared the gene expression profiles between X-ray (8 Gy)-irradiated Ca9-22 cells (an OSCC-derived cell line) and unirradiated Ca9-22 cells. A total of 167 genes with a 2-fold higher level of expression induced by X-ray irradiation were identified. Lipocalin-2 (LCN2) had the greatest increase in expression after X-ray irradiation, and it was categorized in a network that has cancer-related functions with the Ingenuity Pathway Analysis tool. Upregulated expression of LCN2 mRNA was validated by real-time quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis. When the LCN2 gene was knocked down in OSCC cells (Ca9-22 and HSC-2) and lung cancer cells (A549) by using small interfering RNA, the radiosensitivity of these cells was enhanced. Our findings suggest that the overexpression of LCN2 is likely associated with radioresistance in oral cancer and lung cancer cells, and that LCN2 expression levels could be used to predict radioresistance. Thus, regulating the expression or function of LCN2 could enhance the radiation response, resulting in a favorable outcome of radiotherapy.