SEROLOGICAL TYPING OF BRANHAMELLA-CATARRHALIS STRAINS ON THE BASIS OF LIPOPOLYSACCHARIDE ANTIGENS
SEROLOGICAL TYPING OF BRANHAMELLA-CATARRHALIS STRAINS ON THE BASIS OF LIPOPOLYSACCHARIDE ANTIGENS
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DOI：
10.1128/jcm.28.2.182-187.1990
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发表时间：
1990-02-01
影响因子：
9.4
通讯作者：
VANDENABEELE, AM
中科院分区：
文献类型：
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作者：
VANEECHOUTTE, M;VERSCHRAEGEN, G;VANDENABEELE, AM
对来自世界不同地区的302株卡他巴氏杆菌进行了脂多糖抗原性的血清学分型。为此，用下列试剂建立了抑制酶联免疫吸附试验：16个血清型菌株的全菌悬液抗血清和这些菌株通过酚提取法制备的脂多糖。用卡他巴氏杆菌全菌悬液吸附抗血清进行检测。用包被内毒素的微量稀释板，用酶联免疫吸附试验测定血清对同源内毒素的残存活性。活性降低90%的菌株被认为携带与血清型菌株相同的内毒素类型。结果表明，93.4%的受试菌株携带三种可能的内毒素类型之一。根据十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法估计，卡他巴氏杆菌的脂蛋白是粗面型，其表观MR为5500。
A total of 302 strains of Branhamella catarrhalis from different parts of the world were serologically typed according to their lipopolysaccharide (LPS) antigenicity. For this purpose, an inhibition enzyme-linked immunosorbent assay was developed using the following reagents: antisera raised against whole bacterial suspensions for a panel of 16 serotype strains and LPS prepared from these strains by phenol extraction. Antisera were absorbed with whole bacterial suspensions of the B. catarrhalis strains to be tested. The residual activity of the sera against the homologous LPS was determined by means of an enzyme-linked immunosorbent assay, using microdilution plates coated with LPS. Strains which gave > 90% reduction of activity were considered to carry the same LPS type as the serotype strain. It was shown that 93.4% of the strains tested carried one of three possible LPS types. LPS of B. catarrhalis are the rough type and have an apparent Mr of 5,500, as estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.