Studies on peptides. CLXV.1,2) Combination of a new amide-precursor reagent and trimethylsilyl bromide deprotection for the 9-fluorenylmethyloxycarbonyl-based solid-phase synthesis of chicken antral peptide.
Studies on peptides. CLXV.1,2) Combination of a new amide-precursor reagent and trimethylsilyl bromide deprotection for the 9-fluorenylmethyloxycarbonyl-based solid-phase synthesis of chicken antral peptide.
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肽的研究。
DOI：
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发表时间：
1988
影响因子：
1.7
通讯作者：
H. Yajima
中科院分区：
文献类型：
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作者：
L. Guo;S. Funakoshi;N. Fujii;H. Yajima
以新型酰胺前体试剂3-（α-Fmoc-氨基-4-甲氧基苄基）-4-甲氧基苯基丙酸为前体试剂，结合茴香硫醚介导的三甲基溴硅烷脱保护，采用9-芴甲氧羰基（Fmoc）固相合成法合成了鸡胃窦肽的36位氨基酸残基酰胺。使用通过溶液相方法制备的均匀标准样品来检查纯化步骤的有效性。
A36-residue peptide amide corresponding to the entire amino acid sequence of chicken antral peptide was synthesized by the 9-fluorenylmethyloxycarbonyl (Fmoc)-based solid-phase synthesis, for which a new amide precursor reagent, 3-(α-Fmoc-amino-4-methoxybenzyl)-4-methoxyphenylpropionic acid, was employed in combination with thioanisole-mediated trimethylsilyl bromide deprotection. A homogeneous standard sample prepared by the solution-phase method was used to check the effectiveness of the purification step.