INSULIN REGULATION OF RENAL GLUCOSE-METABOLISM IN CONSCIOUS DOGS
INSULIN REGULATION OF RENAL GLUCOSE-METABOLISM IN CONSCIOUS DOGS
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DOI：
10.1172/jci117270
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发表时间：
1994-06-01
影响因子：
15.9
通讯作者：
MILES, JM
中科院分区：
文献类型：
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作者：
CERSOSIMO, E;JUDD, RL;MILES, JM
先前的研究表明，使用净平衡技术时，吸收后肾葡萄糖的产生可以忽略不计，该技术不能区分同时的肾葡萄糖产生和葡萄糖摄取。在狗的左肾静脉和股动脉中手术放置采样导管以及在左肾动脉中放置无阻塞输注导管10天后，通过外周输注[3-H-3]葡萄糖和[2-C-14]甘油来测量全身和肾葡萄糖和甘油动力学。基线测量后，动物接受 2 小时肾内输注胰岛素 (n = 6) 或盐水 (n = 6)。胰岛素组左肾静脉胰岛素浓度从41+/-8增加到92+/-23 pmol/l(P < 0.05)，但动脉胰岛素(接近50 pmol/l)、葡萄糖浓度(接近5.4 mmol/l)或葡萄糖外观(接近18 mu mol . kg(-1) . min(-1))没有变化。左肾葡萄糖摄取量从3.1+/-0.4增加至5.4+/-1.4μmol。千克（-1）。 min(-1) (P < 0.01)，而左肾葡萄糖产量从 2.6+/-0.9 降至 0.7+/-0.5 mu mol 。千克（-1）。胰岛素输注期间分钟(-1) (P < 0.01)。甘油的糖异生作用从 0.23 +/- 0.06 降低至 0.17 +/- 0.04 mu mol 。 kg(-1) min(-1) (P < 0.05) 胰岛素输注期间。这些结果表明，肾葡萄糖的产生和利用约占吸收后犬葡萄糖周转的30%。生理性高胰岛素血症会抑制肾葡萄糖产生并刺激肾葡萄糖摄取约 75%。我们得出的结论是，肾脏对吸收后状态下的全身葡萄糖代谢做出了重大贡献。
Previous studies indicating that postabsorptive renal glucose production is negligible used the net balance technique, which cannot partition simultaneous renal glucose production and glucose uptake. 10 d after surgical placement of sampling catheters in the left renal vein and femoral artery and a nonobstructive infusion catheter in the left renal artery of dogs, systemic and renal glucose and glycerol kinetics were measured with peripheral infusions of [3-H-3]glucose and [2-C-14]glycerol. After baseline measurements, animals received a 2-h intrarenal infusion of either insulin (n = 6) or saline (n = 6). Left renal vein insulin concentration increased from 41+/-8 to 92+/-23 pmol/l(P < 0.05) in the insulin group, but there was no change in either arterial insulin, (similar to 50 pmol/l), glucose concentrations (similar to 5.4 mmol/l), or glucose appearance (similar to 18 mu mol . kg(-1) . min(-1)). Left renal glucose uptake increased from 3.1+/-0.4 to 5.4+/-1.4 mu mol . kg(-1) . min(-1) (P < 0.01) while left renal glucose production decreased from 2.6+/-0.9 to 0.7+/-0.5 mu mol . kg(-1) . min(-1) (P < 0.01) during insulin infusion. Penal gluconeogenesis from glycerol decreased from 0.23 +/- 0.06 to 0.17 +/- 0.04 mu mol . kg(-1) min(-1) (P < 0.05) during insulin infusion.These results indicate that renal glucose production and utilization account for similar to 30% of glucose turnover in postabsorptive dogs. Physiological hyperinsulinemia suppresses renal glucose production and stimulates renal glucose uptake by similar to 75%. We conclude that the kidney makes a major contribution to systemic glucose metabolism in the postabsorptive state.