STRUCTURAL AND FUNCTIONAL ALTERATIONS IN THE GUT OF PARENTERALLY OR ENTERALLY FED RATS
STRUCTURAL AND FUNCTIONAL ALTERATIONS IN THE GUT OF PARENTERALLY OR ENTERALLY FED RATS
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DOI：
10.1016/0022-4804(89)90076-0
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发表时间：
1989-08-01
影响因子：
2.2
通讯作者：
YAMAMOTO, M
中科院分区：
文献类型：
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作者：
HOSODA, N;NISHI, M;YAMAMOTO, M
比较了全肠外营养(TPN)和肠内喂养的不同方案对大鼠小胃肠外营养系统结构和功能的影响。将雄性Wistar大鼠按营养成分和摄入途径随机分为5组：标准饲料组(CE-2组)、低残留饲料组(LRD组)、添加1%(w/v)纤维的LRD组(LRD+纤维组)、基础饲料组(ED组)和TPN组(TPN组)。饲养2周时，回肠末端绒毛高度下降的顺序为：CE-2组和GT组、LRD+纤维组和GT组、LRD组和GT组、ED组和GT组、TPN组。在整个实验期间，CE-2组和LRD+纤维组的粘膜二胺氧化酶活性保持不变。但其余3组粘膜二胺氧化物酶活性明显降低，与结构改变相似，且ED组在2、3、4周时显著降低。血浆二胺氧化酶水平与粘膜二胺氧化酶含量呈正相关，相关系数y=0.20x+0.03，r=0.55(P<；0.01)。这些结果表明，在LRD中添加膳食纤维对肠道结构和功能的维持有良好的效果，血浆二胺氧化酶活性可作为肠内或肠外喂养时小肠功能和/或结构变化的指标。
Various regimens of total parenteral nutrition (TPN) and enteral feeding were compared to determine their effects on the structural and functional changes of rat small intenstine. Male Wistar rats, allocated randomly into five groups on the basis of delivery route and composition of nutrients, were fed as follows: standard rat chow ad libitum (CE-2 group), low-residue diet (LRD group), LRD supplemented with 1% (w/v) fiber (LRD + fiber group), elemental diet (ED group), and TPN (TPN group). At 2 weeks of feeding, villi in the terminal ileum decreased in height in the following order: CE-2 group > LRD + fiber group > LRD group > ED group > TPN group. Mucosal diamine oxidase activity remained unchanged in the CE-2 group and LRD + fiber group throughout the experimental period. However, mucosal diamine oxidase values were significantly lower in the remaining three groups, similar to the structural changes, and those values in the ED group were significantly decreased at 2, 3, and 4 weeks. There was a positive correlation between plasma diamine oxidase level and mucosal diamine oxidase content, with a coefficient correlation of y = 0.20x + 0.03, r = 0.55 (P < 0.01). These results could be interpreted to indicate that addition of dietary fiber to LRD has a favorable effect on the maintenance of intestinal architecture and function during enteral feeding, and plasma diamine oxidase activity can be used as an index of functional and/or structural changes occurring in the small intestine during enteral or parenteral feedings.