Growth inhibitory effect of alk(en)yl thiosulfates derived from onion and garlic in human immortalized and tumor cell lines
Growth inhibitory effect of alk(en)yl thiosulfates derived from onion and garlic in human immortalized and tumor cell lines
复制标题
DOI：
10.1016/j.canlet.2004.10.008
复制
发表时间：
2005-06-01
期刊：
影响因子：
9.7
通讯作者：
Maede, Y
中科院分区：
文献类型：
--
作者：
Chang, HS;Yamato, O;Maede, Y
正丙基硫代硫酸钠(NPTS)和2-丙烯基硫代硫代硫酸钠(2PTS)分别是洋葱和大蒜的天然成分，最初被鉴定为洋葱和大蒜引起狗溶血性贫血的病原体。作为我们对NPTS和2PTS有益功能研究的继续，在本研究中，我们考察了这些化合物的抗肿瘤作用。它们对三种人肿瘤细胞系WiDR、293和HL-60的体外增殖均有抑制作用，且呈剂量依赖关系。总体而言，与2pt相比，NPT似乎具有较弱的抑制细胞生长的活性，但在对这两种化合物都敏感的WiDR细胞中则不然。NPTS和2PTS可致HL-60细胞氧化损伤，诱导细胞凋亡。细胞凋亡的程度与氧化损伤的程度以及这些化合物所引起的细胞毒性大致成正比。这些结果表明，ALK(En)基硫代硫酸盐具有抗肿瘤作用，其机制可能是通过氧化应激诱导细胞凋亡来实现的。(C)2004爱思唯尔爱尔兰有限公司。保留所有权利。
Two alk(en)yl thiosulfates, sodium n-propyl thiosulfate (NPTS) and sodium 2-propenyl thiosulfate (2PTS), are natural constituents of onion and garlic, respectively, which were identified originally as causative agents of onion- and garlic-induced hemolytic anemia in dogs. As a continuation of our studies on the beneficial functions of NPTS and 2PTS, in the present study, we investigated the antitumor effects of these compounds. They were shown to inhibit the in vitro proliferation of three human tumorigenic cell lines, WiDr, 293 and HL-60, in a dose-dependent manner. Overall, NPTS seemed to have weak activity for inhibiting cell growth compared with 2PTS, though not in WiDr cells, which were sensitive to both compounds. NPTS and 2PTS caused oxidative damage to HL-60 cells and induced apoptosis. The extent of apoptosis was approximately proportional to that of the oxidative damage and also to that of the cytotoxicity caused by these compounds. These results suggest that the alk(en)yl thiosulfates have an antitumor effect through the induction of apoptosis initiated by oxidative stress. (c) 2004 Elsevier Ireland Ltd. All rights reserved.