Detection of rabies virus genomic RNA and mRNA in mouse and human brains by using in situ hybridization.
Detection of rabies virus genomic RNA and mRNA in mouse and human brains by using in situ hybridization.
复制标题
使用原位杂交检测小鼠和人脑中狂犬病病毒基因组 RNA 和 mRNA。
DOI：
10.1128/jvi.65.6.2839-2844.1991
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发表时间：
1991
影响因子：
5.4
通讯作者：
Wunner,WH
中科院分区：
文献类型：
--
作者：
Jackson,AC;Wunner,WH
用原位杂交法在小鼠和人脑中检测到狂犬病病毒RNA。制备了针对狂犬病病毒N、NS、M、G和L 5种蛋白的基因组RNA和mRNA3 H标记的单链RNA探针。对狂犬病人和实验感染狂犬病病毒标准株(CVS-11)株和街头狂犬病病毒的小鼠的石蜡包埋脑组织进行了检测。在CVS感染的小鼠中，基因组RNA在感染神经元的核周膜中有多个焦点分布，这可能反映了基因组RNA在病毒工厂的集中。在CVS和街道病毒感染的小鼠脑中，mRNAs比基因组RNAs更为丰富，并且在核膜中呈弥漫性分布。对于编码不同狂犬病病毒蛋白的mRNAs，在受感染的神经元中也存在类似数量的信号。在人类狂犬病病例的脑组织中，基因组RNA比感染神经元中的mRNAs丰富得多。这一发现表明，在死亡期、死后间隔或浸泡固定期间固定剂穿透之前，转录水平上的相对阻断或mRNAs的丢失比基因组RNA的丢失更大。
Rabies virus RNA was detected in mouse and human brains by in situ hybridization. 3H-labeled single-stranded RNA probes were prepared which were specific for genomic RNA and mRNAs coding for the five rabies virus proteins (N, NS, M, G, and L). Paraffin-embedded brain tissues from human cases of rabies and mice experimentally infected with the challenge virus standard (CVS)-11 strain of rabies virus and street rabies virus were examined. In CVS-infected mice, genomic RNA had a multifocal distribution in the perikarya of infected neurons, perhaps reflecting concentration of genomic RNA in viral factories. The mRNAs were more abundant than genomic RNAs in CVS- and street virus-infected mouse brains and had a diffuse distribution in the perikarya. Similar amounts of signal were present in infected neurons for mRNAs coding for different rabies virus proteins. In brain tissues from human cases of rabies, genomic RNA was much more abundant than the mRNAs in infected neurons. This finding suggests either a relative block at the level of transcription or greater loss of mRNAs than of genomic RNA during the agonal period, postmortem interval, or prior to penetration of fixative during immersion fixation.