The effect of antibiotic and probiotic combination therapy on secondary pancreatic infections and oxidative stress parameters in experimental acute necrotizing pancreatitis
The effect of antibiotic and probiotic combination therapy on secondary pancreatic infections and oxidative stress parameters in experimental acute necrotizing pancreatitis
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DOI：
10.1097/00006676-200305000-00009
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发表时间：
2003-05-01
期刊：
影响因子：
2.9
通讯作者：
Kocar, IH
中科院分区：
文献类型：
--
作者：
Akyol, S;Mas, MR;Kocar, IH
简介：环丙沙星和美罗培南对导致胰腺感染的肠道细菌有影响，根据最近的数据，人们认为益生菌，特别是食品工业中使用的益生菌，可以通过抑制细菌来改善预防和治疗继发性胰腺感染的努力。移位。目的：评估单独使用益生菌治疗或联合早期使用两种不同抗生素对血清淀粉酶、胰腺组织病理学、细菌移位和氧化标志物的影响。方法：除假手术组（VI组）外，其余各组均采用3%牛磺胆酸钠（1 mL/kg）诱导大鼠急性胰腺炎模型。稳定期结束后，随机分为7组（n = 20）。在注射后6小时，第1组大鼠接受益生菌布拉酵母菌（25 mg/d，口服q.d.），II组接受美罗培南（60 mg/kg，腹腔注射，每日两次），Ⅲ组腹腔注射环丙沙星40 mg/kg，每日2次，IV组给予相同剂量的益生菌+美罗培南，V组给予益生菌+环丙沙星。VI组（假手术组）和VII组（胰腺炎组）不给予治疗。在诱导后48小时，收集标本。结果和结论：尽管发现治疗组的组织病理学评分低于第VII组，但仅第V组的差异具有统计学显著性（p < 0.001）。在氧化应激的评价中，我们发现，与组VII中的水平相比，处理组中的MDA水平降低，SOD水平升高。单独的益生菌治疗减少了细菌移位。益生菌-抗生素联合治疗可改善组织病理学评分和氧化参数。
Introduction: Ciprofloxacin and meropenem have effects on intestinal bacteria that are responsible for pancreatic infection, and on the basis of recent data it has been argued that probiotics, especially those used in the food industry, could improve efforts to prevent and treat secondary pancreatic infections by inhibiting bacterial translocation. Aims: To evaluate the effects of probiotic treatment alone or in combination with early administration of two different antibiotics on serum amylase, pancreatic histopathology, bacterial translocation, and oxidative markers. Methodology: Acute pancreatitis was induced in rats with 3% sodium taurocholate (1 mL/kg intraductally), except in group VI (sham group). After the stabilization period, the rats were divided into seven groups (n = 20) randomly. At hour 6 after injection, group 1 rats received probiotic Saccharomyces boulardii (25 mg/d orally q.d.), group II received meropenem (60 mg/kg intraperitoneally b.i.d.), group III received ciprofloxacin (40 mg/kg intraperitoneally b.i.d.), group IV received the same dose of probiotic plus meropenem, and group V received probiotic plus ciprofloxacin. Treatment was not given to group VI (sham group) and group VII (pancreatitis group). At hour 48 after induction, specimens were collected. Results and Conclusion: Although histopathologic scores in treatment groups were found to be lower than in group VII, the difference was statistically significant only in group V (p < 0.001). In evaluation of oxidative stress, we found that MDA levels decreased and SOD levels increased in treatment groups in comparison with levels in group VII. Probiotic treatment alone reduced bacterial translocation. Probiotic-antibiotic combination therapy was shown to improve histopathologic scores and oxidative parameters.