Fluid pressure gradients, arising from oscillations in intramedullary pressure, is correlated with the formation of bone and inhibition of intracortical porosity
Fluid pressure gradients, arising from oscillations in intramedullary pressure, is correlated with the formation of bone and inhibition of intracortical porosity
复制标题
DOI：
10.1016/s0021-9290(03)00127-1
复制
发表时间：
2003-10-01
影响因子：
2.4
通讯作者：
Rubin, C
中科院分区：
文献类型：
--
作者：
Qin, YX;Kaplan, T;Rubin, C
由骨组织的功能性负荷产生的液体流动被认为是骨骼质量和形态的关键调节因素。为了验证这一假说，我们研究了在髓内压力的低幅度高频振荡(IMP)的驱动下，骨对生理液体刺激的适应性反应，其中液体压力是在不变形骨组织的情况下获得的。成年火鸡的尺骨通过横切骨进行功能性分离，并将停用四周(n=5)的适应性反应与停用加生理正弦流体压力信号(60 mm Hg，20 Hz)每天10分钟的适应性反应进行比较。单独停用会导致显著的骨丢失(5.7+/-1.9%，p小于或等于0.05)，这是通过骨内吸收导致皮质变薄和皮质内孔隙率增加实现的。通过使骨受到振荡流体流动，骨干中部的骨量显著增加(18.3+/-7.6%，p<；0.05)，骨膜和骨内新骨形成。跨皮质流体压力梯度(Delp(R))是一个与流体速度和流体剪应力密切相关的参数，它在骨干中部的12个等截面上被量化。Delp(R)与总的新骨形成之间有很强的相关性(r=0.75，p=0.01)，Delp(R)与皮质内孔隙率增加的面积呈负相关(r=-0.75，p=0.01)，这表明液体流动信号对于保持骨量和/或抑制骨丢失是必要的，以抵御停用的挑战。通过在没有基质应变的情况下产生这种流体流动，这些数据表明合成代谢流体的运动在建模和重塑过程中起着调节作用。当IMP在骨髓腔内均匀增加时，由于骨的几何和超微结构的不同，流体流动的不同参数有很大的不同，这最终决定了适应过程的空间非均质性。(C)2003爱思唯尔有限公司。保留所有权利。
Fluid flow that arises from the functional loading of bone tissue has been proposed to be a critical regulator of skeletal mass and morphology. To test this hypothesis, the bone adaptive response to a physiological fluid stimulus, driven by low magnitude, high frequency oscillations of intramedullary pressure (ImP), were examined, in which fluid pressures were achieved without deforming the bone tissue. The ulnae of adult turkeys were functionally isolated via transverse epiphyseal osteotomies, and the adaptive response to four weeks of disuse (n = 5) was compared to disuse plus 10 min per day of a physiological sinusoidal fluid pressure signal (60 mmHg, 20 Hz). Disuse alone resulted in significant bone loss (5.7 +/- 1.9%, p less than or equal to 0.05), achieved by thinning the cortex via endosteal resorption and an increase in intracortical porosity. By also subjecting bone to oscillatory fluid flow, a significant increase in bone mass at the mid-diaphysis (18.3 +/- 7.6%, p < 0.05), was achieved by both periosteal and endosteal new bone formation. The spatial distribution of the transcortical fluid pressure gradients (delP(r)), a parameter closely related to fluid velocity and fluid shear stress, was quantified in 12 equal sectors across a section at the mid-diaphyses. A strong correlation was found between the delP(r) and total new bone formation (r = 0.75, p = 0.01); and an inverse correlation (r = -0.75, p = 0.01) observed between delP(r) and the area of increased intracortical porosity, indicating that fluid flow signals were necessary to maintain bone mass and/or inhibit bone loss against the challenge of disuse. By generating this fluid flow in the absence of matrix strain, these data suggest that anabolic fluid movement plays a regulatory role in the modeling and remodeling process. While ImP increases uniformly in the marrow cavity, the distinct parameters of fluid flow vary substantially due to the geometry and ultrastructure of bone, which ultimately defines the spatial non-uniformity of the adaptive process. (C) 2003 Elsevier Ltd. All rights reserved.