An in vivo assessment of the genotoxic potential of bisphenol A and 4-tert-octylphenol in rats
An in vivo assessment of the genotoxic potential of bisphenol A and 4-tert-octylphenol in rats
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DOI：
10.1007/s00204-010-0620-y
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发表时间：
2011-08
影响因子：
6.1
通讯作者：
O. K. Ulutaş;N. Yıldız;E. Durmaz;M. Ahbab;N. Barlas;I. Cok
中科院分区：
文献类型：
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作者：
O. K. Ulutaş;N. Yıldız;E. Durmaz;M. Ahbab;N. Barlas;I. Cok
双酚A（BPA）和辛基酚（OP）是用于制造聚碳酸酯塑料、环氧树脂和非离子表面活性剂的工业化学品。在本研究中，我们使用单细胞凝胶电泳，即所谓的彗星试验，研究了这些化合物在大鼠体内可能的遗传毒性作用。成年雄性Wistar白化病大鼠随机分为6组：BPA 125（接受125 mg/kg bw BPA;n= 6），OP 125（接受125毫克/千克体重OP;n= 6），BPA 250（接受250 mg/kg BPA;n= 6），OP 250（接受250 mg/kg bw OP;n= 6）、对照组（n= 5）和MMS（接受甲磺酸甲酯的阳性对照组;n= 3）。BPA和OP均经口给药4周。对照组也经口接种玉米油4周。评价了彗星参数（包括尾长和尾矩）的可能遗传毒性效应。OP 125和BPA 125与对照组相比，尾长和尾矩无显著差异（P> 0.05）。而OP 250组和BPA 250组的尾长和尾矩与对照组相比有显著性差异（P< 0.05和P < 0.01）。在体内研究了BPA和OP的遗传毒性潜力;需要进一步研究，探索这些化学品在体内遗传毒性潜力的进一步机制。
Bisphenol A (BPA) and octylphenol (OP) are industrial chemicals used in the manufacture of polycarbonate plastics, epoxy resins, and non-ionic surfactants. In the present study, we investigated the possible in vivo genotoxic effects of these compounds in rats using single-cell gel electrophoresis, the so-called comet assay. Adult male Wistar albino rats were divided randomly into six groups as follows: BPA125 (received 125 mg/kg bw BPA;n= 6), OP125 (received 125 mg/kg bw OP;n= 6), BPA250 (received 250 mg/kg BPA;n= 6), OP250 (received 250 mg/kg bw OP;n= 6), control (n= 5), and MMS (positive control group that received methyl methanesulfonate;n= 3). Both BPA and OP were orally administrated for 4 weeks. Controls were orally inoculated with corn oil for 4 weeks as well. Comet parameters including tail length and tail moment were evaluated for possible genotoxic effects. There were no significant differences in the OP125 and in the BPA125 compared with the control group, regarding tail length and tail moment (P> 0.05). However, there were significant differences in the OP250 and in the BPA250 compared with the control group, regarding tail length and tail moment (P< 0.05 andP< 0.01, respectively). The genotoxic potential of BPA and OP was investigated in vivo; there is a need for further studies exploring further mechanisms of the genotoxic potential of these chemicals in vivo.