INDUCTION AND VARIANTS OF NEURONAL NITRIC-OXIDE SYNTHASE TYPE-I DURING SYNAPTOGENESIS
INDUCTION AND VARIANTS OF NEURONAL NITRIC-OXIDE SYNTHASE TYPE-I DURING SYNAPTOGENESIS
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DOI：
10.1096/fasebj.9.9.7541381
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发表时间：
1995-06-01
期刊：
影响因子：
4.8
通讯作者：
SCHMIDT, HHHW
中科院分区：
文献类型：
--
作者：
OGILVIE, P;SCHILLING, K;SCHMIDT, HHHW
在成人中枢神经系统中，一氧化氮（NO）由L-精氨酸通过所谓的组成型或I型NO合酶（NOS-I-155）形成。然而，在发育中的小鼠和大鼠脑中，NOS-I-155免疫反应性和活性的表达较低或不可检测。NOS-I-155在特定脑区的突触发生开始时被急剧诱导，随后是第二阶段，其中特定细胞群和总突触体亚细胞部分中的总NOS-I-155表达降低。此外，短暂观察到NOS-I的两种推定变体：一种具有增加的电泳迁移率的NOS-I免疫反应性蛋白（NOS-I-144）和NOS-I-155对竞争性底物抑制剂N-ω-硝基-L-精氨酸的瞬时超敏性。结论：NOS-I的表达不是组成型的，而是局部诱导的。在中枢神经系统中，这种区域特异性的，出生后的NOS-I诱导的双相模式是一致的一氧化氮在突触发生和突触可塑性的作用。
In the adult central nervous system, nitric oxide (NO) is formed from L-arginine by the so-called constitutive or type I NO synthase (NOS-I-155) However, expression of NOS-I-155 immunoreactivity and activity was low or not detectable in developing mouse and rat brain. NOS-I-155 was sharply induced coincident with the onset of synaptogenesis in specific brain regions, This was followed by a second phase in which total NOS-I-155 expression decreased both in specific cell populations and in the total synaptosomal subcellular fraction, Furthermore, two putative variants of NOS-I were transiently observed: an NOS-I-immunoreactive protein with increased electrophoretic mobility (NOS-I-144) and a transient hypersensitivity of NOS-I-155 to the competitive substrate inhibitor N-omega-nitro-L-arginine. It is concluded that NOS-I expression is not constitutive but locally induced. In the central nervous system, this regionally specific, biphasic pattern of postnatal NOS-I induction is consistent with a role for NO in synaptogenesis and synaptic plasticity.