Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis.
Serological activity of a characteristic phenolic glycolipid from Mycobacterium leprae in sera from patients with leprosy and tuberculosis.
复制标题
麻风病和结核病患者血清中麻风分枝杆菌特征性酚糖脂的血清学活性。
DOI：
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发表时间：
1983
影响因子：
4.6
通讯作者：
R. Rees
中科院分区：
文献类型：
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作者：
S. Brett;P. Draper;S. Payne;R. Rees
用免疫扩散和酶联免疫吸附试验(EL ISA)研究了麻风分枝杆菌酚性糖脂的血清学活性，该糖脂可从麻风分枝杆菌感染的鼹鼠肝脏中大量获得。总体上，两种方法之间具有良好的相关性，但酶联免疫吸附试验更为敏感和简便。麻风患者血清中抗糖脂抗体水平较高。然而，与粗制超声波的抗体滴度相比，糖脂的抗体滴度较低。由于糖脂大量存在，这表明它的免疫原性不是很强。抗糖脂抗体，特别是IgM类抗体，在某些类结核麻风患者中可见，尽管效价远低于麻风血清中的滴度。治疗5年以上皮肤涂片阴性的麻风患者，其血清中抗M。麻风提取的糖脂活性明显降低。肺结核患者血清中抗Ig G和Ig M糖脂抗体水平与正常对照组无显著差异。感染堪萨斯分枝杆菌、禽分枝杆菌或细胞内分枝杆菌的患者的糖脂抗体水平也落在正常健康人的范围内。
Serological activity against a purified phenolic glycolipid from Mycobacterium leprae, which may be obtained in large amounts from M. leprae infected armadillo liver, was investigated using immunodiffusion and an enzyme linked immunosorbent assay (ELISA). Generally a good correlation was obtained between these techniques, but the ELISA was more sensitive and convenient. Relatively high IgG and IgM anti-glycolipid antibody levels were found in lepromatous leprosy patients. The antibody titres to the glycolipid were, however, low when compared with antibody titres to crude sonicates. Since the glycolipid is present in large quantities, this suggests that it is not very immunogenic. Antibody against the glycolipid especially of the IgM class, was demonstrable in some tuberculoid leprosy patients, although at much lower titres than in the lepromatous leprosy sera. In lepromatous leprosy patients that were skin smear negative after more than 5 years of treatment the IgG anti-M. leprae derived glycolipid activity had decreased markedly. The anti-IgG and IgM glycolipid antibody levels in tuberculosis patients did not differ significantly from the levels in appropriate normal healthy subjects. The glycolipid antibody levels in patients infected with M. kansasii, M. avium or M. intracellulare also fell within the range of normal healthy individuals.