A graphene-enhanced molecular beacon for homogeneous DNA detection
A graphene-enhanced molecular beacon for homogeneous DNA detection
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用于同质 DNA 检测的石墨烯增强分子信标。
DOI：
10.1039/b9nr00401g
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发表时间：
2010-01-01
期刊：
影响因子：
6.7
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Li, Fan;Huang, Yan;Fan, Chunhai
在这项工作中，我们报道了一种新的基于石墨烯的分子信标（MB）的设计，它可以灵敏地和选择性地检测特定的DNA序列。水溶性氧化石墨烯（GO）分化发夹和双链DNA的能力为DNA检测提供了一种新的方法。我们发现在氧化石墨烯的存在下，MB的背景荧光被显著抑制，这增加了信本比，从而提高了灵敏度。此外，发夹DNA的单错配分化能力保持不变，使得该方法具有较高的选择性。
In this work, we report the design of a novel graphene-based molecular beacon (MB) that could sensitively and selectively detect specific DNA sequences. The ability of water-soluble graphene oxide (GO) to differentiated hairpin and dsDNA offered a new approach to detect DNA. We found that the background fluorescence of MB was significantly suppressed in the presence of GO, which increased the signal-to-background ratio, hence the sensitivity. Moreover, the single-mismatch differentiation ability of hairpin DNA was maintained, leading to high selectivity of this new method.