Patterns of gene expression during Drosophila mesoderm Development
Patterns of gene expression during Drosophila mesoderm Development
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DOI：
10.1126/science.1062660
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发表时间：
2001-08-31
期刊：
影响因子：
56.9
通讯作者：
Scott, MP
中科院分区：
文献类型：
--
作者：
Furlong, EEM;Andersen, EC;Scott, MP
转录因子扭曲启动果蝇中胚层发育，从而形成心脏，体细胞肌肉和其他细胞类型。我们使用果蝇胚胎分道机，分离出足够的纯合扭曲突变体胚胎以执行DNA微阵列实验。在不同的发育阶段比较了扭曲功能丧失胚胎，具有无处不在扭曲表达的胚胎和野生型胚胎的转录曲线。结果暗示了数百个基因，许多基因具有脊椎动物同源物，在中胚层发育的阶段特定过程中。一种与脊椎动物Gli基因有关的基因，Gleeful，对于体细胞发育至关重要，足以使神经细胞表达肌肉标记。
The transcription factor Twist initiates Drosophila mesoderm development, resulting in the formation of heart, somatic muscle, and other cell types. Using a Drosophila embryo sorter, we isolated enough homozygous twist mutant embryos to perform DNA microarray experiments. Transcription profiles of twist loss-of-function embryos, embryos with ubiquitous twist expression, and wild-type embryos were compared at different developmental stages. The results implicate hundreds of genes, many with vertebrate homologs, in stage-specific processes in mesoderm development. One such gene, gleeful, related to the vertebrate Gli genes, is essential for somatic muscle development and sufficient to cause neural cells to express a muscle marker.