Modulation of Leishmania (L.) amazonensis Growth in Cultured Mouse Macrophages by Prostaglandins and Platelet Activating Factor.
Modulation of Leishmania (L.) amazonensis Growth in Cultured Mouse Macrophages by Prostaglandins and Platelet Activating Factor.
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DOI：
10.1155/s0962935194000177
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发表时间：
1994
影响因子：
4.6
通讯作者：
Jancar S
中科院分区：
文献类型：
--
作者：
Lonardoni MV;Barbieri CL;Russo M;Jancar S
研究了内源性血小板活化因子（PAF）和白藜芦醇苷（Arylandins）在Letsbmanta（L.）研究了亚马逊河流域，以及这些炎症介质的作用与一氧化氮产生之间的可能相关性。研究发现，用10 - 5 M PAF拮抗剂BN-52021或WEB-2086预处理巨噬细胞，分别使巨噬细胞感染增加17%和59%。环加氧酶抑制剂吲哚美辛（10 μg/ml）可引起明显的抑制作用，而加入PGE（10 ~（-3）M）则可逆转这种抑制作用。这些结果表明，PAF抑制了利什曼原虫对巨噬细胞的感染，而Escherlandin则增强了这种感染，并且巨噬细胞在这种感染过程中产生了这些介质。在感染前向培养基中加入这些介质证实了这一点; PAF（10−6、10−9和10− 12 M）显著降低了感染，而PGE 2（10−5 M）诱导了显著增强。外源性PAF对巨噬细胞感染的这种影响被逆转的两个PAF拮抗剂在本研究中使用，以及由一氧化氮合成的抑制剂，L-精氨酸甲酯（100 mM）。两者合计的数据表明，内源性生产的PAF和PGE 2发挥相反的作用Lesbmana-巨噬细胞的相互作用，一氧化氮可能参与增强破坏的PAF诱导的寄生虫。
The role of endogenously synthesized PAF and prostaglandins on the infection of mouse macrophages by Letsbmanta (L.) amazonensis was investigated, as well as the possible correlation between the effects of these inflammatory mediators with nitric oxide production. It was found that pretreatment of macrophages with 10−5 M of the PAF antagonists, BN-52021 or WEB-2086, increased macrophage infection by 17 and 59%, respectively. The cyclooxygenase inhibitor, indomethacin (10 μg/ml), induced a significant inhibition which was reversed by addition of PGE (10-3 M) to the culture medium. These results suggested that the infection of macrophages by leisbmanla is inhibited by PAF and enhanced by prostaglandins and that these mediators are produced by macrophages during this infection. This was confirmed by addition of these mediators to the culture medium before infection; PAF (10−6, 10−9 and 10−12M) reduced significantly the infection whereas PGE2 (10−5 M) induced a marked enhancement. This effect of exogenous PAF on macrophage infection was reversed by the two PAF antagonists used in this study as well as by the inhibitor of nitric oxide synthesis, L-arginine methyl ester (100 mM). Taken together the data suggest that endogenous production of PAF and PGE2 exert opposing effects on Lesbmana–macrophage interaction and that nitric oxide may be involved in the augmented destruction of parasites induced by PAF.