Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs
Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs
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DOI：
10.1111/j.1365-2052.2009.01952.x
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发表时间：
2010-02-01
期刊：
影响因子：
2.4
通讯作者：
Jorgensen, C. B.
中科院分区：
文献类型：
--
作者：
Jacobsen, M.;Kracht, S. S.;Jorgensen, C. B.
产肠毒素大肠杆菌F4ab/Ac引起的小肠感染是养猪业的主要福利问题和经济负担。对这种感染的自然抗性是作为孟德尔隐性性状遗传的，目前丹麦养猪业在选择计划中使用了MUC4基因中分离敏感性/抗性的多态。为了阐明猪对大肠杆菌F4ab/Ac易感的遗传背景，建立了猪候选区域的详细单倍型图谱。该区域覆盖约3.7Mb。研究使用的材料是一个三代家庭，创始人是两头野猪和八头大白母猪。使用粘附法对所有猪进行了F4ab/Ac敏感性的表型鉴定。它们的单倍型是通过侧翼标记的分离分析得知的。通过有针对性的方法，对候选区域进行多态筛选，主要集中在内含子序列上。共测定了18个基因的部分序列，并在GP5、CENTB2、APOD、PCYT1A、OSTpha、ZDHHC19、TFRC、AcK1、MUC4、MUC20、KIAA0226、LRCH3和MUC13中发现了多态性。总体而言，在创始人一代中发现了227个多态。分析显示，在MUC4周围有一个大的单倍型区块，跨越至少1.5Mb，与F4ab/Ac易感性有关。
P>Infection of the small intestine by enterotoxigenic Escherichia coli F4ab/ac is a major welfare problem and financial burden for the pig industry. Natural resistance to this infection is inherited as a Mendelian recessive trait, and a polymorphism in the MUC4 gene segregating for susceptibility/resistance is presently used in a selection programme by the Danish pig breeding industry. To elucidate the genetic background involved in E. coli F4ab/ac susceptibility in pigs, a detailed haplotype map of the porcine candidate region was established. This region covers approximately 3.7 Mb. The material used for the study is a three generation family, where the founders are two Wild boars and eight Large White sows. All pigs have been phenotyped for susceptibility to F4ab/ac using an adhesion assay. Their haplotypes are known from segregation analysis using flanking markers. By a targeted approach, the candidate region was subjected to screening for polymorphisms, mainly focusing on intronic sequences. A total of 18 genes were partially sequenced, and polymorphisms were identified in GP5, CENTB2, APOD, PCYT1A, OSTalpha, ZDHHC19, TFRC, ACK1, MUC4, MUC20, KIAA0226, LRCH3 and MUC13. Overall, 227 polymorphisms were discovered in the founder generation. The analysis revealed a large haplotype block, spanning at least 1.5 Mb around MUC4, to be associated with F4ab/ac susceptibility.