Evidence of a mate-finding cue in the hermaphrodite nematode Caenorhabditis elegans
Evidence of a mate-finding cue in the hermaphrodite nematode Caenorhabditis elegans
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DOI：
10.1073/pnas.032225799
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发表时间：
2002-02-05
影响因子：
11.1
通讯作者：
Sternberg, PW
中科院分区：
文献类型：
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作者：
Simon, JM;Sternberg, PW
当秀丽隐杆线虫的雄性与它们的雌雄同体的同伴交往时，它们停止觅食行为，开始交配。在这里，我们详细介绍了几种用于证明可扩散线索与此过程相关的检测方法。这种暗示是两性异形的，只由雌雄同体发出，只在男性中引起反应。男性被吸引到，经常反向运动，并保持在地区的琼脂条件与雌雄同体。从我们的研究中，我们提出了一种形式的运动，其工作原理是吸引男性到他们的交配伙伴从远处和功能，一旦男性到达，在举行吸引男性在近距离。雌雄同体的外阴并不需要提示。来自一般感觉突变体osm-5和osm-6的雄性对信号没有反应，而雄性特异性突变体lov-1和pkd-2有反应。最后，雄性从多个分离的线虫也响应类似的这种线索表明，这种线索是强大的，并一直保持在最近的进化。
When males of the roundworm Caenorhabditis elegans come into association with their hermaphroditic counterparts they cease foraging behavior and begin to mate. Here we detail several assays used to demonstrate that a diffusible cue is correlated with this process. This cue is sexually dimorphic, given off only by the hermaphrodite and eliciting a response only in the male. Males are attracted to, reverse direction of movement frequently, and remain in regions of agar conditioned with hermaphrodites. From our studies we suggest a form of kinesis that works by attracting males to their mating partners from a distance and functions, once males arrive, in holding attracted males in close proximity. The hermaphrodite vulva is not required for the cue. Males from general sensory mutants osm-5 and osm-6 fail to respond to the cue, whereas male-specific mutants lov-1 and pkd-2 respond. Finally, that males from multiple isolates of C elegans also respond similarly to this cue indicates that this cue is robust and has been maintained during recent evolution.