Changes in superoxide dismutase mRNA expression by streptozotocin-induced diabetes
Changes in superoxide dismutase mRNA expression by streptozotocin-induced diabetes
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DOI：
10.1111/j.1476-5381.1996.tb15712.x
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发表时间：
1996-10-01
影响因子：
7.3
通讯作者：
Kobayashi, T
中科院分区：
文献类型：
--
作者：
Kamata, K;Kobayashi, T
1 实验旨在研究超氧阴离子在链脲佐菌素 (STZ) 诱导的糖尿病大鼠主动脉内皮依赖性舒张减弱中的作用。2 用去甲肾上腺素 (NA, 5 x 10(-8) 预收缩的主动脉螺旋条中对乙酰胆碱 (ACh, 10(-7) M) 的内皮依赖性舒张反应类似于 3 x 10(-7) M)在STZ诱导的糖尿病大鼠中显着降低。 STZ诱导的糖尿病大鼠单次给予ACh后舒张恢复期较对照血管更快。 3 主动脉条与超氧化物歧化酶(SOD, 60 u ml(-1))预孵育使单次给予ACh后糖尿病主动脉舒张恢复期正常化，而过氧化氢酶(150 u ml(-1))或吲哚美辛(10(-5) M)对糖尿病主动脉舒张恢复期没有影响。 4 SOD (180 u ml(-1)) 引起 NA 预收缩的主动脉条舒张，与年龄匹配的对照血管相比，糖尿病主动脉中 SOD 诱导的舒张程度明显更大。 5 当观察糖尿病主动脉中 Mn-SOD 或 Cu-Zn-SOD mRNA 表达的变化时，Mn-SOD mRNA 表达显着下降，Cu-Zn-SOD 略有下降。 6 这些结果表明，糖尿病主动脉中 Mn-SOD 或 Cu-Zn-SOD mRNA 表达明显下降，而 Cu-Zn-SOD 略有下降。 STZ诱导的糖尿病大鼠中可能发生超氧阴离子对NO的影响，这可能是由于Mn-SOD或Cu-Zn-SOD mRNA表达降低所致。
1 Experiments were designed to investigate the involvement of superoxide anions in the attenuated endothelium-dependent relaxation of the rat aorta from streptozotocin (STZ)-induced diabetic rats.2 The endothelium-dependent relaxation responses to acetylcholine (ACh, 10(-7) M) in helical strips of the aorta precontracted with noradrenaline (NA, 5 x 10(-8) similar to 3 x 10(-7) M) were significantly decreased in STZ-induced diabetic rats. The recovery phase of the relaxation after single administration of ACh in the STZ-induced diabetic rats was more rapid than those in control vessels.3 Preincubation of aortic strips with superoxide dismutase (SOD, 60 u ml(-1)) normalized the recovery phase of the relaxation of diabetic aorta after single administration of ACh, whereas catalase (150 u ml(-1)) or indomethacin (10(-5) M) had no effects on the relaxation.4 SOD (180 u ml(-1)) caused relaxation in NA precontracted aortic strips and the degree of the SOD-induced relaxation was significantly greater in diabetic aorta as compared with age-matched control vessels.5 When the changes in mRNA expressions of Mn-SOD or Cu-Zn-SOD were observed, Mn-SOD mRNA expression was markedly decreased, and Cu-Zn-SOD was slightly decreased in diabetic aorta.6 These results suggest that the rapid destruction of NO by superoxide anions may occur in the STZ-induced diabetic rats, and this may be due to a decrease in mRNA expression of Mn-SOD or Cu-Zn-SOD.