Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus
Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus
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DOI：
10.1016/j.biortech.2010.03.006
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发表时间：
2010-08-01
影响因子：
11.4
通讯作者：
Zhang, Yuanxing
中科院分区：
文献类型：
--
作者：
Cai, Menghao;Zhou, Xiangshan;Zhang, Yuanxing
研究了柠檬酸盐对海洋真菌Aspergillus glaucus HB 1 -19的促生作用及其对该菌生长和性发育的影响。在琼脂平板培养中，15 mM柠檬酸降低菌落径向生长和曲霉素A生产分别为31.5%和23.0%。对有性闭囊壳的形成有360%的促进作用，但对无性分生孢子的产生有84.8%的抑制作用。在液体培养中，添加40 mM柠檬酸可使曲霉A的产量提高80.0%，生物量增加16.7%，糖利用率提高10.0%。柠檬酸钠的作用不明显，甚至相反。柠檬酸和低pH能显著促进丙酮酸的积累，但抑制琥珀酸和富马酸的产生。此外，低pH有利于柠檬酸盐的利用。有机酸的变化与曲霉素A的生物合成密切相关。与pH对照相比，柠檬酸的作用包括pH降低引发和柠檬酸盐利用增强。(C)2010爱思唯尔有限公司版权所有。
Aspergiolide A production enhancement by citrate and its effects on growth and sexual development of marine-derived fungus Aspergillus glaucus HB1-19 were investigated. In agar plate culture, 15 mM citric acid decreased colony radial growth and aspergiolide A production by 31.5% and 23.0%, respectively. It also improved sexual cleistothecium formation by 360% but depressed asexual conidiospore generation by 84.8%. In submerged culture, adding 40 mM citric acid finally promoted aspergiolide A production by 80.0%, which accompanied with 16.7% increase of biomass and 10.0% enhancement of sugar utilization. Differently, sodium citrate made no obvious or even opposite effects. Citrate and low pH could significantly improve pyruvate accumulation but inhibit succinate and fumarate production. Moreover, low pH was favorable to citrate utilization. Organic acids changes were closely related to aspergiolide A biosynthesis. Comparing to pH controls, effects of citric acid comprised pH decrease solicitation and citrate utilization enhancement. (C) 2010 Elsevier Ltd. All rights reserved.