Isolation and culture of Schwann cells.
Isolation and culture of Schwann cells.
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DOI：
10.1007/978-1-62703-444-9_9
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Tao, Yanmei
中科院分区：
文献类型：
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作者：
Tao, Yanmei
原代培养的雪旺细胞对于研究调节雪旺细胞增殖、存活、分化和髓鞘形成的分子机制以及开发有效移植以再生受损脊髓或周围神经系统至关重要。在这里，我们描述了从新生大鼠或小鼠中分离和纯化原代施万细胞的基本方案，并讨论了一些适合成年神经培养的修改和施万细胞富集的可选方法。
Primarily cultured Schwann cells are essential for the investigation of molecular mechanisms regulating proliferation, survival, differentiation, and myelination of Schwann cell and for the development of efficient transplantation for regeneration of injured spinal cord or peripheral nervous system. Here we describe a basic protocol for isolation and purification of primary Schwann cell from neonatal rat or mouse and discuss some modifications adapted to the culturing from adult nerves and optional methods for Schwann cell enrichment.