HISTAMINE INHIBITS DOPAMINE RELEASE IN THE MOUSE STRIATUM VIA PRESYNAPTIC-H3 RECEPTORS
HISTAMINE INHIBITS DOPAMINE RELEASE IN THE MOUSE STRIATUM VIA PRESYNAPTIC-H3 RECEPTORS
复制标题
DOI：
10.1007/bf01244933
复制
发表时间：
1993-01-01
期刊：
影响因子：
--
通讯作者：
GOTHERT, M
中科院分区：
文献类型：
--
作者：
SCHLICKER, E;FINK, K;GOTHERT, M
在[H-3]多巴胺25 nmol/l预孵育的灌流小鼠纹状体脑片中，组胺10 mumol/l可抑制电（3 Hz）诱发的氚溢出18%。氟哌啶醇使抑制程度增加至38%，但不受（1）阿托品、（2）将刺激频率降低至0.3 Hz或（3）将[H-3]多巴胺（用于预孵育）浓度增加至100 nmol/l的影响。组胺的作用可被H-3受体激动剂R-（-）-α-甲基组胺所模拟;它不受H-1受体拮抗剂二甲茚和H-2受体拮抗剂雷尼替丁的影响，但可被H-3受体拮抗剂硫代哌丁胺所消除。氚溢出诱发的Ca 2+离子（引入到Ca 2 +-免费，K+-丰富的培养基中含有河豚毒素）不受组胺10 μ mol/L的情况下，但抑制（30%）在氟哌啶醇的存在下，组胺的效果被废除硫代哌丁胺。 总之，小鼠纹状体多巴胺能神经末梢具有突触前H-3受体。多巴胺自身受体的同时阻断增加了H-3受体介导的多巴胺释放抑制的程度。
In superfused mouse striatal slices preincubated with [H-3]dopamine 25 nmol/l, the electrically (3 Hz) evoked tritium overflow was inhibited by histamine 10 mumol/l by 18%. The degree of inhibition was increased to 38% by haloperidol but not affected by (1) atropine, (2) reducing the stimulation frequency to 0.3 Hz or (3) increasing the concentration of [H-3]dopamine (used for preincubation) to 100 nmol/l. The effect of histamine was mimicked by the H-3 agonist R-(-)-alpha-methylhistamine; it was not affected by the H-1 antagonist dimetindene and the H-2 antagonist ranitidine but abolished by the H-3 antagonist thioperamide. Tritium overflow evoked by Ca2+ ions (introduced into Ca2+-free, K+-rich medium containing tetrodotoxin) was not affected by histamine 10 mumol/l in the absence, but inhibited (by 30%) in the presence of haloperidol; the effect of histamine was abolished by thioperamide. In conclusion, the dopaminergic nerve terminals in the mouse striatum are endowed with presynaptic H-3 receptors. Simultaneous blockade of dopamine autoreceptors increases the extent of the H-3 receptor-mediated inhibition of dopamine release.