Alteration of mitochondrial genomes containing atpA genes in the sexual progeny of cybrids between Raphanus sativus cms line and Brassica napus cv. Westar
Alteration of mitochondrial genomes containing atpA genes in the sexual progeny of cybrids between Raphanus sativus cms line and Brassica napus cv. Westar
复制标题
DOI：
10.1007/bf00227405
复制
发表时间：
1992-09
影响因子：
5.4
通讯作者：
T. Sakai;J. Imamura
中科院分区：
文献类型：
--
作者：
T. Sakai;J. Imamura
我们研究了x射线照射的油菜（cms系）和碘乙酸酰胺处理的油菜（brassica napuscv）原生质体“供体-受体”融合产生的杂交体线粒体基因组的命运。西星。10个融合产物中有2个是雄性不育的，染色体数目为b的二倍体。显著。以豌豆线粒体atp酶亚基基因（atpA）为探针，采用DNA-DNA杂交技术对杂交后代及其线粒体基因组进行分析。其中一个杂种18-3具有3.0 kb的b片段特征。将bamhi酶切的DNA与探针杂交，得到一个2.0 kb的非亲本片段。在该杂种的第一次回交后代中，杂交模式遗传不稳定。一个4.0 kb的萝卜片段在杂交中未检测到，出现在bc1代的一个兄弟姐妹中，而2.0 kb的非亲本片段在另一个兄弟姐妹中丢失。杂交组合12-9的bc1后代兄弟姐妹的杂交模式也各不相同。杂交后代mtDNA的改变持续到bc2代。杂交18-3的mt基因组中没有明显的异质状态或亚化学计量分子。讨论了在mt基因组中观察到的改变的可能原因。
We have investigated the fate of the mitochondrial genomes of cybrids derived from “donor-recipient” protoplast fusion between X-irradiatedRaphanus sativus(cms line) and iodoacetamide-treatedBrassica napuscv. Westar. Two out of ten fusion products were male-sterile with the diploid chromosome number ofB. napus. The mitochondrial (mt) genomes of the cybrids and their progeny were further analyzed by DNA-DNA hybridizaion using the pea mitochondrial ATPase subunit gene (atpA) as a probe. One cybrid, 18-3, had a 3.0 kb fragment characteristic ofB. napusand a 2.0 kb non-parental fragment when theBamHI-digested DNA was hybridized with the probe. In the first-backcrossed progeny of this cybrid, the hybridization pattern was not stably inherited. A 4.0 kb radish fragment, not detectable in the cybrid, appeared in one of the BC1generation siblings, and the 2.0 kb non-parental fragment was lost in another. The hybridization patterns in BC1progeny siblings of cybrid 12-9 were also varied. The alteration of mtDNA in the cybrid progeny continued to the BC2generation. There was no clear evidence of a heteroplasmic state or of sub-stoichiometric molecules in the mt genome of cybrid 18-3. A possible cause of the observed alteration in the mt genome is discussed.