Transcriptome of Paeonia ostii developing seeds revealed transcriptional factors, mRNAs and LncRNAs related to fatty acid synthesis and accumulation
Transcriptome of Paeonia ostii developing seeds revealed transcriptional factors, mRNAs and LncRNAs related to fatty acid synthesis and accumulation
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DOI：
10.1016/j.indcrop.2022.115670
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发表时间：
2022-11
影响因子：
5.9
通讯作者：
Yanchao Yuan;Hua Sun;Xinyu Wang;Linqiang Gao;Chunying Liu;S. Gai;Yuxi Zhang
中科院分区：
文献类型：
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作者：
Yanchao Yuan;Hua Sun;Xinyu Wang;Linqiang Gao;Chunying Liu;S. Gai;Yuxi Zhang
由于其高水平的不饱和脂肪酸，特别是α-亚麻酸，奥氏亚麻籽是有利的植物油的极好来源。探索植物油脂合成和积累的调控网络，有利于提高植物油脂含量和产量。孔籽在本研究中，在脂肪酸（FA）含量的增加和稳定阶段的种子进行了评估和采样的比较转录组分析在P。奥斯蒂。共筛选出740个差异表达基因，其中包含362个转录因子，这些基因与油脂代谢、碳分配和油脂生物合成的能量供应有关。此外，还获得了26对与FA合成和积累相关的LncRNA-mRNA。关键基因（LTP、ADH、ACP、PDAT、FAD 2、FAD 3、WRI 1、FUS 3、LEC 1等）的表达。并使用qRT-PCR验证了八种推定的LncRNA。通过异位过表达、双荧光素酶报告基因检测和酵母单杂交等方法，进一步研究PoWRI 1和PoPDAT的功能和相互关系。结果表明，PoWRI 1和PoPDAT的过表达促进了FA的积累。研究结果可为牡丹基因组的互补、油脂积累的分子调控机制的研究和高油新品种的培育提供参考。
Paeonia ostiiseeds are an excellent source of favorable vegetable oil due to their high levels of unsaturated fatty acids, especially alpha-linolenic acid. The exploration of regulatory networks for lipid synthesis and accumulation is beneficial for increasing the lipid content and yield ofP. ostiiseeds. In the present study, seeds at the increasing and stable stages of fatty acid (FA) content were evaluated and sampled for comparative transcriptome analysis inP. ostii. Totally, 740 significantly differential expression genes containing 362 TFs were screened and were relevant for lipid metabolism, carbon partitioning, and energy provision for oil biosynthesis. Moreover, 26 pairs of LncRNA-mRNA associated with FA synthesis and accumulation were obtained. The expression of key genes (LTP,ADH,ACP,PDAT,FAD2,FAD3,WRI1,FUS3,LEC1, etc.) and eight putative LncRNAs were validated using qRT-PCR. Furthermore, the function and relationship ofPoWRI1andPoPDATwere determined through ectopic overexpression, dual-luciferase reporter assay, and yeast one hybrid. The results showed that the over-expression ofPoWRI1andPoPDATpromoted FA accumulation. Together, the results obtained could provide a reference for the genomic complement and studying the molecular regulatory mechanism of oil accumulation and cultivating new varieties with high oil seeds in tree peony.