Rapid new bone tissue remodeling during distraction osteogenesis is associated with apoptosis
Rapid new bone tissue remodeling during distraction osteogenesis is associated with apoptosis
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DOI：
10.1016/s0736-0266(02)00097-9
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发表时间：
2003-01-01
影响因子：
2.8
通讯作者：
Simpson, H
中科院分区：
文献类型：
--
作者：
Li, G;Dickson, GR;Simpson, H
在牵引成骨过程中，新骨形成并经历快速重塑。细胞凋亡可能是牵张成骨过程中控制多余愈伤组织去除的调节机制之一。使用兔胫骨延长模型并以0.7毫米/天的速度延长3周。通过透射电子显微镜 (TEM) 和末端脱氧核苷酸转移酶介导的 dUTP-生物素缺口末端标记 (TUNEL) 方法检查来自牵引间隙的再生组织的细胞凋亡变化。通过抗酒石酸酸性磷酸酶（TRAP）染色证明破骨细胞骨吸收活性。凋亡细胞主要存在于矿化前沿的纤维组织与新生骨的过渡区域，以及再生体中心附近的新生骨表面或附近。在截骨骨端附近的成熟骨中，TUNEL 标记大大减少。 TEM检查证实在再生的各个区域存在具有凋亡变化的细胞。 TRAP染色显示，再生体中破骨细胞骨吸收活性的分布模式与TUNEL标记的分布模式相似。凋亡细胞在再生不同区域的定位以及破骨细胞的活性表明细胞凋亡与牵张成骨过程中的骨形成和重塑密切相关。 (C) 2002 年骨科研究学会。由爱思唯尔科学有限公司出版。保留所有权利。
During the process of distraction osteogenesis new bone forms and undergoes rapid remodeling. Apoptosis may be one of the regulatory mechanisms governing the removal of the redundant callus during distraction osteogenesis. A rabbit tibial lengthening model was used and lengthened at 0.7 mm/day for 3 weeks. The regenerating tissues from the distraction gap were examined for apoptotic changes by transmission electron microscopy (TEM) and the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method. Osteoclastic bone resorption activities were demonstrated by tartrate resistant acid phosphatase (TRAP) staining. The apoptotic cells were mainly present in the transitional regions between the fibrous tissue and the new bone in the mineralization front, and close to or on the new bone surfaces near the center of the regenerate. The TUNEL labeling was greatly reduced in the mature bone near the osteotomied bone ends. TEM examination confirmed the presence of cells with apoptotic changes at various regions of the regenerate. TRAP staining revealed that osteoclastic bone resorption activities in the regenerate were in a similar pattern of distribution to those of the TUNEL labeling. The localization of apoptotic cells at the different regions of the regenerate, accompanied by the osteoclast activities, suggest that apoptosis is closely related to bone formation and remodeling during distraction osteogenesis. (C) 2002 Orthopaedic Research Society. Published by Elsevier Science Ltd. All rights reserved.