Effect of Ocimum basilicum on apoptosis in testis of rats after exposure to electromagnetic field
Effect of Ocimum basilicum on apoptosis in testis of rats after exposure to electromagnetic field
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DOI：
10.5897/ajpp11.384
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发表时间：
2011-09-01
影响因子：
--
通讯作者：
Khaki, Amir Afshin
中科院分区：
文献类型：
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作者：
Khaki, Arash;Fathiazad, Fatemeh;Khaki, Amir Afshin
罗勒（Ocimum basilicum）的药用可以追溯到伊朗、中国和印度的古代。这种草药自古以来就被用作药物和食物，众所周知，O。罗勒对精子发生有益，因此推测这种草药也可能对精子参数提供保护。将雄性Wistar大鼠（n = 40）分为4组，对照组（n = 10）和3个处理组（n = 30）。第一治疗组给予O.罗勒提取物组（1.5g/kg体重），第二提取物组（1.5g/kg体重）接受O.罗勒提取物（1.5 g/kg体重）和电磁场（EMF）暴露在50赫兹连续40天，而第三组只接受电磁场暴露连续40天。将动物保持在标准条件下。试验期结束时，从所有组成员中取出大鼠睾丸组织。进行组织制备并通过TUNEL法分析细胞凋亡。电磁场组细胞凋亡率明显高于其他各组（P
Medicinal use of basil, Ocimum basilicum, dates to ancient times in Iran, China and India. This herb has been used since ancient times as a medicine and food and it is known that the antioxidant effect of O. basilicum is beneficial to spermatogenesis, so it was hypothesized that this herb might also provide protection to sperm parameters. Male Wistar rats (n = 40) were allocated to four groups, a control group (n = 10) and three treatment groups (n = 30). The first treatment group received O. basilicum extract (1.5 g/kg body weight), the second extract group received O. basilicum extract (1.5 g/kg body weight) and electromagnetic field (EMF) exposure at 50 Hz for 40 consecutive days, whilst the third group received only EMF exposure for 40 consecutive days. Animals were maintained under standard conditions. At the conclusion of the test period, rat testes tissues were removed from all group members. Tissue preparation was performed and analyzed for apoptosis by TUNEL method. There was a significant increase in apoptosis in EMF group when compared with other groups (P