Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening.
Introducing Discrete Frequency Infrared Technology for High-Throughput Biofluid Screening.
复制标题
DOI：
10.1038/srep20173
复制
发表时间：
2016-02-04
影响因子：
4.6
通讯作者：
Baker MJ
中科院分区：
文献类型：
--
作者：
Hughes C;Clemens G;Bird B;Dawson T;Ashton KM;Jenkinson MD;Brodbelt A;Weida M;Fotheringham E;Barre M;Rowlette J;Baker MJ
准确的早期诊断对患者的生存、管理和生活质量至关重要。生物流体是早期诊断的关键，因为它们易于收集和密切参与人体功能。干燥流体沉积物的大规模中红外成像为生物医学实验室提供了高通量分子分析范例。令人兴奋的可调谐量子级联激光器的出现允许收集离散频率红外数据，从而实现临床相关的时间尺度。通过扫描目标频率的光谱质量，再现性和诊断潜力，可以保持，同时显着减少采集时间和处理要求，采样16个血清点，分别为0.6，5.1和15%的相对标准偏差（RSD）为199，14和9个离散频率。我们使用这种可重复的方法来证明概念快速诊断;来自脑癌、乳腺癌、肺癌和皮肤癌患者的40个独特的干燥液体活检组织在2.4秒的累积时间内与10个非癌症对照进行了分类，准确率高达90%。
Accurate early diagnosis is critical to patient survival, management and quality of life. Biofluids are key to early diagnosis due to their ease of collection and intimate involvement in human function. Large-scale mid-IR imaging of dried fluid deposits offers a high-throughput molecular analysis paradigm for the biomedical laboratory. The exciting advent of tuneable quantum cascade lasers allows for the collection of discrete frequency infrared data enabling clinically relevant timescales. By scanning targeted frequencies spectral quality, reproducibility and diagnostic potential can be maintained while significantly reducing acquisition time and processing requirements, sampling 16 serum spots with 0.6, 5.1 and 15% relative standard deviation (RSD) for 199, 14 and 9 discrete frequencies respectively. We use this reproducible methodology to show proof of concept rapid diagnostics; 40 unique dried liquid biopsies from brain, breast, lung and skin cancer patients were classified in 2.4 cumulative seconds against 10 non-cancer controls with accuracies of up to 90%.